IED ID | IndEnz0011000105 |
Enzyme Type ID | glucanase000105 |
Protein Name |
Endoglucanase 1 EC 3.2.1.4 Cellulase 1 Endo-1,4-beta-glucanase 1 Endoglucanase I |
Gene Name | CEL7B |
Organism | Humicola insolens (Soft-rot fungus) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Sordariomycetidae Sordariales Chaetomiaceae Humicola Humicola insolens (Soft-rot fungus) |
Enzyme Sequence | QKPGETKEVHPQLTTFRCTKRGGCKPATNFIVLDSLSHPIHRAEGLGPGGCGDWGNPPPKDVCPDVESCAKNCIMEGIPDYSQYGVTTNGTSLRLQHILPDGRVPSPRVYLLDKTKRRYEMLHLTGFEFTFDVDATKLPCGMNSALYLSEMHPTGAKSKYNPGGAYYGTGYCDAQCFVTPFINGLGNIEGKGSCCNEMDIWEANSRASHVAPHTCNKKGLYLCEGEECAFEGVCDKNGCGWNNYRVNVTDYYGRGEEFKVNTLKPFTVVTQFLANRRGKLEKIHRFYVQDGKVIESFYTNKEGVPYTNMIDDEFCEATGSRKYMELGATQGMGEALTRGMVLAMSIWWDQGGNMEWLDHGEAGPCAKGEGAPSNIVQVEPFPEVTYTNLRWGEIGSTYQELQ |
Enzyme Length | 402 |
Uniprot Accession Number | P56680 |
Absorption | |
Active Site | ACT_SITE 197; /note=Nucleophile; ACT_SITE 202; /note=Proton donor |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
DNA Binding | |
EC Number | 3.2.1.4 |
Enzyme Function | FUNCTION: The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (2); Beta strand (25); Chain (1); Disulfide bond (9); Glycosylation (2); Helix (12); Modified residue (1); Turn (5) |
Keywords | 3D-structure;Carbohydrate metabolism;Cellulose degradation;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Pyrrolidone carboxylic acid;Secreted |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
Modified Residue | MOD_RES 1; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000269|PubMed:9335168 |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | X-ray crystallography (8) |
Cross Reference PDB | 1A39; 1DYM; 1OJI; 1OJJ; 1OJK; 2A39; 6YOZ; 6YP1; |
Mapped Pubmed ID | 12890535; 34458755; |
Motif | |
Gene Encoded By | |
Mass | 44,577 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |