Detail Information for IndEnz0011000112
IED ID IndEnz0011000112
Enzyme Type ID glucanase000112
Protein Name Endoglucanase EG-II
EGII
EC 3.2.1.4
Cellulase
Endo-1,4-beta-glucanase
Gene Name egl2
Organism Hypocrea jecorina (Trichoderma reesei)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Hypocreaceae Trichoderma Hypocrea jecorina (Trichoderma reesei)
Enzyme Sequence MNKSVAPLLLAASILYGGAVAQQTVWGQCGGIGWSGPTNCAPGSACSTLNPYYAQCIPGATTITTSTRPPSGPTTTTRATSTSSSTPPTSSGVRFAGVNIAGFDFGCTTDGTCVTSKVYPPLKNFTGSNNYPDGIGQMQHFVNEDGMTIFRLPVGWQYLVNNNLGGNLDSTSISKYDQLVQGCLSLGAYCIVDIHNYARWNGGIIGQGGPTNAQFTSLWSQLASKYASQSRVWFGIMNEPHDVNINTWAATVQEVVTAIRNAGATSQFISLPGNDWQSAGAFISDGSAAALSQVTNPDGSTTNLIFDVHKYLDSDNSGTHAECTTNNIDGAFSPLATWLRQNNRQAILTETGGGNVQSCIQDMCQQIQYLNQNSDVYLGYVGWGAGSFDSTYVLTETPTSSGNSWTDTSLVSSCLARK
Enzyme Length 418
Uniprot Accession Number P07982
Absorption
Active Site ACT_SITE 239; /note="Proton donor/acceptor"; /evidence="ECO:0000305|PubMed:21898652, ECO:0000305|PubMed:8093602"; ACT_SITE 350; /note="Nucleophile"; /evidence="ECO:0000269|PubMed:8093602, ECO:0000305|PubMed:21898652"
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; Evidence={ECO:0000269|PubMed:3356188, ECO:0000269|PubMed:3384334};
DNA Binding
EC Number 3.2.1.4
Enzyme Function FUNCTION: Endoglucanase (EG) that cleaves the internal beta-1,4-glucosidic bonds in cellulose (PubMed:3384334). The degradation of cellulose involves an interplay between different cellulolytic enzymes. Hydrolysis starts with EGs, which cut internal glycosidic linkages to reduce the polymerization degree of the substrate and creates new chain ends for exocellobiohydrolases (CBHs). The CBH release the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain. Finally, beta-1,4-glucosidases hydrolyze the cellobiose and other short cello-oligosaccharides into glucose units (Probable). {ECO:0000269|PubMed:3384334, ECO:0000305}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Beta strand (12); Chain (1); Disulfide bond (4); Domain (1); Glycosylation (1); Helix (11); Modified residue (1); Region (3); Signal peptide (1); Turn (6)
Keywords 3D-structure;Carbohydrate metabolism;Cellulose degradation;Direct protein sequencing;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Pyrrolidone carboxylic acid;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:3384334}.
Modified Residue MOD_RES 22; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000269|PubMed:3384334
Post Translational Modification
Signal Peptide SIGNAL 1..21; /evidence=ECO:0000269|PubMed:3384334
Structure 3D X-ray crystallography (1)
Cross Reference PDB 3QR3;
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 44,227
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda