IED ID | IndEnz0011000113 |
Enzyme Type ID | glucanase000113 |
Protein Name |
Endoglucanase 4 EC 3.2.1.4 Endo-1,4-beta glucanase 4 |
Gene Name | At1g23210 F26F24.6 T26J12.2 |
Organism | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
Enzyme Sequence | MAGKSFMTPAIMLAMLLLISPETYAGHDYRDALRKSILFFEGQRSGKLPPDQRLKWRRDSALRDGSSAGVDLTGGYYDAGDNVKFGFPMAFTTTMMSWSVIDFGKTMGPELENAVKAIKWGTDYLMKATQIPDVVFVQVGDAYSDHNCWERPEDMDTLRTVYKIDKDHSGSEVAGETAAALAAASIVFEKRDPVYSKMLLDRATRVFAFAQKYRGAYSDSLYQAVCPFYCDFNGYEDELLWGAAWLHKASKKRVYREFIVKNQVILRAGDTIHEFGWDNKHAGINVLVSKMVLMGKAEYFQSFKQNADEFICSLLPGISHPQVQYSQGGLLVKSGGSNMQHVTSLSFLLLTYSNYLSHANKVVPCGEFTASPALLRQVAKRQVDYILGDNPMKMSYMVGYGSRFPQKIHHRGSSVPSVVDHPDRIGCKDGSRYFFSNNPNPNLLIGAVVGGPNITDDFPDSRPYFQLTEPTTYINAPLLGLLGYFSAHY |
Enzyme Length | 489 |
Uniprot Accession Number | O49296 |
Absorption | |
Active Site | ACT_SITE 81; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10140; ACT_SITE 409; /evidence=ECO:0000255|PROSITE-ProRule:PRU10059; ACT_SITE 460; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060; ACT_SITE 469; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
DNA Binding | |
EC Number | 3.2.1.4 |
Enzyme Function | |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (4); Chain (1); Glycosylation (1); Signal peptide (1) |
Keywords | Carbohydrate metabolism;Cell wall biogenesis/degradation;Cellulose degradation;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..25; /evidence=ECO:0000255 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | 14576160; 16720694; 17687051; 18667720; 18775970; |
Motif | |
Gene Encoded By | |
Mass | 54,630 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |