| IED ID | IndEnz0011000141 |
| Enzyme Type ID | glucanase000141 |
| Protein Name |
Lichenase-2 EC 3.2.1.73 1- 3,1- 4 -beta-glucanase isoenzyme EII Endo-beta-1,3-1,4 glucanase II Lichenase II Fragment |
| Gene Name | |
| Organism | Hordeum vulgare (Barley) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Pooideae Triticodae Triticeae Hordeinae Hordeum Hordeum vulgare (Barley) |
| Enzyme Sequence | PPSVESIGVCYGMSANNLPAASTVVSMFKFNGIKSMRLYAPNQAALQAVGGTGINVVVGAPNDVLSNLAASPAAAASWVKSNIQAYPKVSFRYVCVGNEVAGGATRNLVPAMKNVHGALVAAGLGHIKVTTSVSQAILGVFSPPSAGSFTGEAAAFMGPVVQFLARTNAPLMANIYPYLAWAYNPSAMDMGYALFNASGTVVRDGAYGYQNLFDTTVDAFYTAMGKHGGSSVKLVVSESGWPSGGGTAATPANARFYNQHLINHVGRGTPRHPGAIETYIFAMFNENQKDSGVEQNWGLFYPNMQHVYPINF |
| Enzyme Length | 312 |
| Uniprot Accession Number | P12257 |
| Absorption | |
| Active Site | ACT_SITE 99; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O22317; ACT_SITE 238; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O22317 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.; EC=3.2.1.73; |
| DNA Binding | |
| EC Number | 3.2.1.73 |
| Enzyme Function | FUNCTION: Functions in plant cell wall hydrolysis during mobilization of the endosperm in germinating grain or during the growth of vegetative tissues. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan metabolism; beta-D-glucan degradation. |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (14); Chain (1); Glycosylation (1); Helix (15); Non-terminal residue (1); Signal peptide (1); Turn (4) |
| Keywords | 3D-structure;Direct protein sequencing;Glycoprotein;Glycosidase;Hydrolase;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL <1..6; /evidence=ECO:0000269|Ref.2 |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 1AQ0; 1GHR; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 32,774 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.73; |