| IED ID | IndEnz0011000142 |
| Enzyme Type ID | glucanase000142 |
| Protein Name |
Probable glucan endo-1,3-beta-glucosidase A6 EC 3.2.1.39 1- 3 -beta-glucan endohydrolase 1- 3 -beta-glucanase Anther-specific protein A6 Beta-1,3-endoglucanase Protein MATERNAL EFFECT EMBRYO ARREST 48 |
| Gene Name | A6 MEE48 At4g14080 dl3080c |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MSLLAFFLFTILVFSSSCCSATRFQGHRYMQRKTMLDLASKIGINYGRRGNNLPSPYQSINFIKSIKAGHVKLYDADPESLTLLSQTNLYVTITVPNHQITALSSNQTIADEWVRTNILPYYPQTQIRFVLVGNEILSYNSGNVSVNLVPAMRKIVNSLRLHGIHNIKVGTPLAMDSLRSSFPPSNGTFREEITGPVMLPLLKFLNGTNSYFFLNVHPYFRWSRNPMNTSLDFALFQGHSTYTDPQTGLVYRNLLDQMLDSVLFAMTKLGYPHMRLAISETGWPNFGDIDETGANILNAATYNRNLIKKMSASPPIGTPSRPGLPIPTFVFSLFNENQKSGSGTQRHWGILHPDGSPIYDVDFTGQTPLTGFNPLPKPTNNVPYKGQVWCVPVEGANETELEETLRMACAQSNTTCAALAPGRECYEPVSIYWHASYALNSYWAQFRNQSIQCFFNGLAHETTTNPGNDRCKFPSVTL |
| Enzyme Length | 478 |
| Uniprot Accession Number | Q06915 |
| Absorption | |
| Active Site | ACT_SITE 135; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O22317; ACT_SITE 280; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O22317 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.; EC=3.2.1.39; |
| DNA Binding | |
| EC Number | 3.2.1.39 |
| Enzyme Function | FUNCTION: Probable beta-1,3-glucanase that may be involved in the degradation of callose walls around the microspore tetrad during pollen development (Probable). May be required for pollen exine formation (PubMed:21849515). {ECO:0000269|PubMed:21849515, ECO:0000305|PubMed:8281185}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (1); Signal peptide (1) |
| Keywords | Disulfide bond;Glycosidase;Hydrolase;Plant defense;Reference proteome;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Contains two additional disulfide bonds, but it is unclear if they are between the pairs Cys-409-Cys-416 and Cys-425-Cys-471 or between the pairs Cys-409-Cys-471 and Cys-416-Cys-425. {ECO:0000250|UniProtKB:Q94G86}. |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12777046; 15100403; 16831835; 17496121; 18036205; 18433503; 26216374; 32854314; |
| Motif | |
| Gene Encoded By | |
| Mass | 53,472 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |