| IED ID | IndEnz0011000148 |
| Enzyme Type ID | glucanase000148 |
| Protein Name |
Endo-1,3-1,4-beta-glycanase ExoK EC 3.2.1.- Succinoglycan biosynthesis protein ExoK |
| Gene Name | exoK RB1080 SMb20955 |
| Organism | Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Alphaproteobacteria Hyphomicrobiales Rhizobiaceae Sinorhizobium/Ensifer group Sinorhizobium Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti) Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) |
| Enzyme Sequence | MTIDRYRRFARLAFIATLPLAGLATAAAAQEGANGKSFKDDFDTLDTRVWFVSDGWNNGGHQNCTWSKKQVKTVDGILELTFEEKKVKERNFACGEIQTRKRFGYGTYEARIKAADGSGLNSAFFTYIGPADKKPHDEIDFEVLGKNTAKVQINQYVSAKGGNEFLADVPGGANQGFNDYAFVWEKNRIRYYVNGELVHEVTDPAKIPVNAQKIFFSLWGTDTLTDWMGTFSYKEPTKLQVDRVAFTAAGDECQFAESVACQLERAQSE |
| Enzyme Length | 269 |
| Uniprot Accession Number | P33693 |
| Absorption | |
| Active Site | ACT_SITE 138; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10064; ACT_SITE 142; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10064 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.2.1.- |
| Enzyme Function | FUNCTION: Cleaves high molecular weight succinoglycan to yield LMW succinoglycan. Dynamically regulates the molecular weight distribution of succinoglycan by cleaving nascent succinoglycan only during a limited period after its synthesis, perhaps before it undergoes a time-dependent change in its conformation or aggregation state. {ECO:0000269|PubMed:11902715, ECO:0000269|PubMed:9560202}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan metabolism; exopolysaccharide biosynthesis. {ECO:0000269|PubMed:9560202}. |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Domain (1); Signal peptide (1) |
| Keywords | Exopolysaccharide synthesis;Glycosidase;Hydrolase;Plasmid;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9560202}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..29; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | Plasmid pSymB,megaplasmid 2 |
| Mass | 30,084 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |