| IED ID | IndEnz0011000155 |
| Enzyme Type ID | glucanase000155 |
| Protein Name |
Cellulase 1 EC 3.2.1.4 Avicelase Endo-1,4-beta-glucanase Endoglucanase |
| Gene Name | cel1 |
| Organism | Streptomyces reticuli |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces reticuli |
| Enzyme Sequence | MKRRTTAVLTLTALLGTALTALPVQQAGAEEVEQVRNGTFDTTTDPWWTSNVTAGLSDGRLCADVPGGTTNRWDSAIGQNDITLVKGETYRFSFHASGIPEGHVVRAVVGLAVSPYDTWQEASPVLTEADGSYSYTFTAPVDTTQGQVAFQVGGSTDAWRFCVDDVSLLGGVPPEVYEPDTGPRVRVNQVAYLPAGPKNATLVTDATARLPWQLRNAQGTTVARGLTVPRGVDASSGQNVHSIDFGSYRGRGTGYTLVADGETSHPFDIDAAAYRPLRLDSVKYYYTQRSGIAIRDDLRPGYGRAAGHLNVAPNQGDANVPCQPGVCDYTLDVTGGWYDAGDHGKYVVNGGIATWELLSTYERSLTARTGHPAALGDGTLALPESGNKVPDVLDEARWELEFLLKMQVPAGQPLAGMAHHKLHDEQWTGLPLLPDQDPQKRELHPPTTAATLNLAATAAQAARLYRPFDKAFAARALTAARTAWQAALAHPDLLADPNDGTGGGAYNDDDVTDEFYWAAAELYLTTGERQFADHVLDSPVHTADIFGPTGFDWGHTAAAGRLDLALVPSRLPGRDQVRRSVIKAADTYLATLTAHPYGMPYAPAGNRYDWGSSHQVLNNGVVLASAYDLTGAAKYRDGALQGMDYVLGRNALNMSYVTGYGEVSSHNQHSRWYAHQLDPTLPNPPSGTLAGGPNSSIQDPYAQSKLTGCVGQFCYIDDIQSWSTNETAINWNAALARMASFAADQG |
| Enzyme Length | 746 |
| Uniprot Accession Number | Q05156 |
| Absorption | |
| Active Site | ACT_SITE 342; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10140; ACT_SITE 669; /evidence=ECO:0000255|PROSITE-ProRule:PRU10059; ACT_SITE 717; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060; ACT_SITE 726; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | FUNCTION: This endoglucanase acts only on crystalline cellulose. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (4); Chain (1); Domain (1); Region (2); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cellulose degradation;Direct protein sequencing;Glycosidase;Hydrolase;Polysaccharide degradation;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Note=Exists in both mycelium-associated and extracellular forms. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..29; /evidence=ECO:0000269|PubMed:1282194 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 80,000 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |