| IED ID | IndEnz0011000182 |
| Enzyme Type ID | glucanase000182 |
| Protein Name |
Probable glucan 1,3-beta-glucosidase D EC 3.2.1.58 Exo-1,3-beta-glucanase D |
| Gene Name | exgD AO090001000604 |
| Organism | Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus oryzae (Yellow koji mold) Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Enzyme Sequence | MPSHSRSRDRYRSERDPSRRYREVYDDDDDDDFDYHPRERRRYRRDDYQHDIRSHESPNYNDDLNEYDAAAEDPAVPLRSHDVEGRRRERSRAGESPIASPSRRDRNRGGEEYRRHGTYGDGGSPTRAMRDRRHRSRDGQRARPRDMDREARRQRRRERARGAAAMKHKSSDSTNSGSHLLSADALAKLRSHYDEEDQRERSQEQEQPRMESKRQRKRPIVGDEPQALAPFPDETPRGQSKGRIVSGAYLEEGHPEMEVRHRGGGGPAMEARWRKEGNWDGTMEGSDAQPPFWKRKKWWIVIGVLVVVLAIVIPVAVVMSKKHGHDDDKSGSSSSVDNSDSPYISSLDGLSHDSIPESAQGSILDPWTWYDTRDFNLTFTNETVGGLPIMGLNSTWDDSTRPNDNVPPLNESFPYGSQPIRGVNLGGWLSIEPFIVPSLFENYSSKDRIIDEYTLCKKLGSSAASTIEKHYADFISEQDFIDMRDAGLDHVRIQFSYWAVTTYDDDPYVAKISWRYLLRAIEYCRKYGLRVNLDPHGIPGSQNGWNHSGREGVIGWLNGTDGQLNRQRSLDFHNQISQFFAQPRYKNVVTIYGLVNEPLMLSLPVEDVLNWTTDATKLVQKNGISAYVTVHDGFLNLSKWKQMLKDRPDRMFLDTHQYTIFNTGQIVLNHTDRVKLICNDWYNMIKEINTTSAGWGPTICGEWSQADTDCAQYLNNVGRGTRWEGTFAIGDSTVYCPTADTGPTCSCASANAPPADYSDGYKKFLQTYAEAQMSAFGTAQGWFYWTWHTESAAQWSYKTAWKNGYMPKKAYAPDFKCGDDIPSFGDLPEYY |
| Enzyme Length | 831 |
| Uniprot Accession Number | Q2UMV7 |
| Absorption | |
| Active Site | ACT_SITE 597; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 702; /note=Nucleophile; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose.; EC=3.2.1.58; |
| DNA Binding | |
| EC Number | 3.2.1.58 |
| Enzyme Function | FUNCTION: Glucosidase involved in the degradation of cellulosic biomass. Active on lichenan (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (5); Erroneous initiation (1); Glycosylation (11); Region (2); Topological domain (2); Transmembrane (1) |
| Keywords | Carbohydrate metabolism;Cell membrane;Cell wall biogenesis/degradation;Glycoprotein;Glycosidase;Hydrolase;Membrane;Polysaccharide degradation;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 95,127 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |