IED ID | IndEnz0011000188 |
Enzyme Type ID | glucanase000188 |
Protein Name |
Cell surface mannoprotein MP65 EC 3.2.1.- Mannoprotein of 65 kDa Soluble cell wall protein 10 |
Gene Name | MP65 CMP65 SCW1 SCW10 CAALFM_C210030CA CaO19.1779 CaO19.9345 |
Organism | Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Debaryomycetaceae Candida/Lodderomyces clade Candida Candida albicans (Yeast) Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) |
Enzyme Sequence | MLFKSFVTFTVLANALAAPLAHQHHQHKEEKRAVHVVTTTNVVVVTIGNGDQTTTFAAPSVAAESSVSVSVNTEPPQNHPTTTQDVASASTYPSSTDGSAASSSAAASSSSQAGSEPSGGVGSGGAKGITYSPYSDNGGCKSESQIASEIAQLSGFDVIRLYGVDCSQVEAVLKAKTSSQKIFAGIFDVSSITSGIESLAEAVKSCGSWDDIYTVSIGNELVNAGSATPSQIKAYVEEGRKALKAAGYTGPVVSVDTFIAVINNPDLCDYSDYMAVNAHAFFDGHVVAENSGAWVLQQIQRVWTACGGKKNVLITETGWPSRGDSNGVAVPSKSNQQAAISSIKSSCGASAILFTAFNDLWKADGPYNAEKYWGIYSN |
Enzyme Length | 378 |
Uniprot Accession Number | Q59XX2 |
Absorption | |
Active Site | ACT_SITE 316; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O22317 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | |
DNA Binding | |
EC Number | 3.2.1.- |
Enzyme Function | FUNCTION: Surface mannoprotein required for hyphal morphogenesis, surface adherence, and pathogenicity. Contributes in a high proportion to the carbohydrate component of the matrix due to high levels of glycosylation and may play important roles during biofilm development and maintenance. Acts as a major antigen target of host cell-mediated immune response. Induces extensive T-cell proliferation of human peripheral blood mononuclear cells. Facilitates host dendritic cells maturation and promotes cytokine production through its glycosylated portion while its protein core is essentially involved in induction of T-cell response. {ECO:0000269|PubMed:17217426}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (1); Chain (1); Compositional bias (1); Region (1); Signal peptide (1) |
Keywords | Cell adhesion;Cell wall;Cell wall biogenesis/degradation;Direct protein sequencing;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal;Virulence |
Interact With | |
Induction | INDUCTION: Expressed predominantly by the mycelial cells. Also induced during cell wall regeneration and biofilm formation. Repressed by fluconazole. {ECO:0000269|PubMed:16455273, ECO:0000269|PubMed:21622905, ECO:0000269|PubMed:7738727}. |
Subcellular Location | SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000269|PubMed:15302828, ECO:0000269|PubMed:17277107, ECO:0000269|PubMed:17905924, ECO:0000269|PubMed:19098294, ECO:0000269|PubMed:21622905, ECO:0000269|PubMed:8698482}. |
Modified Residue | |
Post Translational Modification | PTM: Glycosylated protein with a polysaccharide moiety composed exclusively of mannose and glucose at a ratio of 12.7 to 1. Contributes highly to the carbohydrate component of the matrix. Treatment with tunicamycin impairs glycosylation. {ECO:0000269|PubMed:19098294}. |
Signal Peptide | SIGNAL 1..32; /evidence=ECO:0000269|PubMed:8698482 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 39,264 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |