Detail Information for IndEnz0011000188
IED ID IndEnz0011000188
Enzyme Type ID glucanase000188
Protein Name Cell surface mannoprotein MP65
EC 3.2.1.-
Mannoprotein of 65 kDa
Soluble cell wall protein 10
Gene Name MP65 CMP65 SCW1 SCW10 CAALFM_C210030CA CaO19.1779 CaO19.9345
Organism Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Debaryomycetaceae Candida/Lodderomyces clade Candida Candida albicans (Yeast) Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Enzyme Sequence MLFKSFVTFTVLANALAAPLAHQHHQHKEEKRAVHVVTTTNVVVVTIGNGDQTTTFAAPSVAAESSVSVSVNTEPPQNHPTTTQDVASASTYPSSTDGSAASSSAAASSSSQAGSEPSGGVGSGGAKGITYSPYSDNGGCKSESQIASEIAQLSGFDVIRLYGVDCSQVEAVLKAKTSSQKIFAGIFDVSSITSGIESLAEAVKSCGSWDDIYTVSIGNELVNAGSATPSQIKAYVEEGRKALKAAGYTGPVVSVDTFIAVINNPDLCDYSDYMAVNAHAFFDGHVVAENSGAWVLQQIQRVWTACGGKKNVLITETGWPSRGDSNGVAVPSKSNQQAAISSIKSSCGASAILFTAFNDLWKADGPYNAEKYWGIYSN
Enzyme Length 378
Uniprot Accession Number Q59XX2
Absorption
Active Site ACT_SITE 316; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O22317
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.2.1.-
Enzyme Function FUNCTION: Surface mannoprotein required for hyphal morphogenesis, surface adherence, and pathogenicity. Contributes in a high proportion to the carbohydrate component of the matrix due to high levels of glycosylation and may play important roles during biofilm development and maintenance. Acts as a major antigen target of host cell-mediated immune response. Induces extensive T-cell proliferation of human peripheral blood mononuclear cells. Facilitates host dendritic cells maturation and promotes cytokine production through its glycosylated portion while its protein core is essentially involved in induction of T-cell response. {ECO:0000269|PubMed:17217426}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Compositional bias (1); Region (1); Signal peptide (1)
Keywords Cell adhesion;Cell wall;Cell wall biogenesis/degradation;Direct protein sequencing;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal;Virulence
Interact With
Induction INDUCTION: Expressed predominantly by the mycelial cells. Also induced during cell wall regeneration and biofilm formation. Repressed by fluconazole. {ECO:0000269|PubMed:16455273, ECO:0000269|PubMed:21622905, ECO:0000269|PubMed:7738727}.
Subcellular Location SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000269|PubMed:15302828, ECO:0000269|PubMed:17277107, ECO:0000269|PubMed:17905924, ECO:0000269|PubMed:19098294, ECO:0000269|PubMed:21622905, ECO:0000269|PubMed:8698482}.
Modified Residue
Post Translational Modification PTM: Glycosylated protein with a polysaccharide moiety composed exclusively of mannose and glucose at a ratio of 12.7 to 1. Contributes highly to the carbohydrate component of the matrix. Treatment with tunicamycin impairs glycosylation. {ECO:0000269|PubMed:19098294}.
Signal Peptide SIGNAL 1..32; /evidence=ECO:0000269|PubMed:8698482
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 39,264
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda