IED ID | IndEnz0011000189 |
Enzyme Type ID | glucanase000189 |
Protein Name |
Probable endo-beta-1,4-glucanase celB Endoglucanase celB EC 3.2.1.4 Carboxymethylcellulase celB Cellulase B |
Gene Name | celB NFIA_114250 |
Organism | Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Fumigati Aspergillus fischeri Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus) |
Enzyme Sequence | MALTLAATALVLLPLVTAQQIGSIAENHPELTTYRCSSQAGCVAQSTSVVLDINAHWIHQNGAQTSCTTSSGLDPSLCPDKVTCSQNCVVEGITDYSSFGVQNSGDAMTLRQYQVQNGQIKTLRPRVYLLAEDGINYSKLQLLNQEFTFDVDASKLPCGMNGALYLSEMDASGGRSALNPAGATYGTGYCDAQCFNPGPWINGEANTAGAGACCQEMDLWEANSRSTIFSPHPCTTAGLYACTGAECYSICDGYGCTYNPYELGAKDYYGYGLTIDTAKPITVVTQFMTADNTATGTLAEIRRLYVQDGKVIGNTAVAMTEAFCSSSRTFEELGGLQRMGEALGRGMVPVFSIWDDPGLWMHWLDSDGAGPCGNTEGDPAFIQANYPNTAVTFSKVRWGDIGSTYSS |
Enzyme Length | 407 |
Uniprot Accession Number | A1D932 |
Absorption | |
Active Site | ACT_SITE 216; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 221; /note=Proton donor; /evidence=ECO:0000250 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
DNA Binding | |
EC Number | 3.2.1.4 |
Enzyme Function | FUNCTION: Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates (By similarity). {ECO:0000250}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (2); Chain (1); Glycosylation (1); Signal peptide (1) |
Keywords | Carbohydrate metabolism;Cellulose degradation;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 43,421 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |