| IED ID | IndEnz0011000196 |
| Enzyme Type ID | glucanase000196 |
| Protein Name |
Beta-glucanase EC 3.2.1.73 1,3-1,4-beta-D-glucan 4-glucanohydrolase Endo-beta-1,3-1,4 glucanase Lichenase |
| Gene Name | |
| Organism | Paenibacillus macerans (Bacillus macerans) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Paenibacillaceae Paenibacillus Paenibacillus macerans (Bacillus macerans) |
| Enzyme Sequence | MKKKSCFTLVTTFAFSLIFSVSALAGSVFWEPLSYFNRSTWEKADGYSNGGVFNCTWRANNVNFTNDGKLKLGLTSSAYNKFDCAEYRSTNIYGYGLYEVSMKPAKNTGIVSSFFTYTGPAHGTQWDEIDIEFLGKDTTKVQFNYYTNGVGGHEKVISLGFDASKGFHTYAFDWQPGYIKWYVDGVLKHTATANIPSTPGKIMMNLWNGTGVDDWLGSYNGANPLYAEYDWVKYTSN |
| Enzyme Length | 237 |
| Uniprot Accession Number | P23904 |
| Absorption | |
| Active Site | ACT_SITE 128; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10064; ACT_SITE 132; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10064 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.; EC=3.2.1.73; |
| DNA Binding | |
| EC Number | 3.2.1.73 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (18); Chain (1); Disulfide bond (1); Domain (1); Helix (5); Mutagenesis (1); Signal peptide (1) |
| Keywords | 3D-structure;Disulfide bond;Glycosidase;Hydrolase;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (10) |
| Cross Reference PDB | 1AJK; 1AJO; 1AXK; 1BYH; 1CPM; 1CPN; 1GLH; 1MAC; 1U0A; 2AYH; |
| Mapped Pubmed ID | 16483609; 7852389; 7937966; 8200344; 9489923; 9618460; |
| Motif | |
| Gene Encoded By | |
| Mass | 26,589 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |