| IED ID | IndEnz0011000206 |
| Enzyme Type ID | glucanase000206 |
| Protein Name |
Endoglucanase-6B EC 3.2.1.4 Cellulase 6B Endo-1,4-beta-glucanase 6B |
| Gene Name | cel6B |
| Organism | Humicola insolens (Soft-rot fungus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Sordariomycetidae Sordariales Chaetomiaceae Humicola Humicola insolens (Soft-rot fungus) |
| Enzyme Sequence | QSGNPFSGRTLLVNSDYSSKLDQTRQAFLSRGDQTNAAKVKYVQEKVGTFYWISNIFLLRDIDVAIQNARAAKARGENPIVGLVLYNLPDRDCSAGESSGELKLSQNGLNRYKNEYVNPFAQKLKAASDVQFAVILEPDAIGNMVTGTSAFCRNARGPQQEAIGYAISQLQASHIHLYLDVANGGWLGWADKLEPTAQEVATILQKAGNNAKIRGFSSNVSNYNPYSTSNPPPYTSGSPSPDESRYATNIANAMRQRGLPTQFIIDQSRVALSGARSEWGQWCNVNPAGFGQPFTTNTNNPNVDAIVWVKPGGESDGQCGMGGAPAAGMWFDAYAQMLTQNAHDEIAR |
| Enzyme Length | 348 |
| Uniprot Accession Number | Q7SIG5 |
| Absorption | |
| Active Site | ACT_SITE 92; /note="Proton donor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10057, ECO:0000303|PubMed:10794732"; ACT_SITE 139; /note="Proton donor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10057, ECO:0000269|PubMed:10794732" |
| Activity Regulation | |
| Binding Site | BINDING 52; /note=Substrate; /evidence=ECO:0000250|UniProtKB:Q9C1S9; BINDING 54; /note=Substrate; /evidence=ECO:0000250|UniProtKB:Q9C1S9; BINDING 183; /note=Substrate; /evidence=ECO:0000250|UniProtKB:Q9C1S9; BINDING 186; /note=Substrate; /evidence=ECO:0000250|UniProtKB:Q9C1S9; BINDING 222; /note=Substrate; /evidence=ECO:0000250|UniProtKB:Q9C1S9; BINDING 282; /note=Substrate; /evidence=ECO:0000250|UniProtKB:Q9C1S9; BINDING 310; /note=Substrate; /evidence=ECO:0000250|UniProtKB:Q9C1S9; BINDING 314; /note=Substrate; /evidence=ECO:0000250|UniProtKB:Q9C1S9 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; Evidence={ECO:0000269|PubMed:9335167}; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | FUNCTION: Plays a central role in the recycling of plant biomass. The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (12); Binding site (8); Chain (1); Helix (16); Region (1); Turn (3) |
| Keywords | 3D-structure;Carbohydrate metabolism;Cellulose degradation;Glycosidase;Hydrolase;Polysaccharide degradation |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 1DYS; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 37,753 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |