| IED ID | IndEnz0011000222 |
| Enzyme Type ID | glucanase000222 |
| Protein Name |
Probable endo-beta-1,4-glucanase B Endoglucanase B EC 3.2.1.4 Carboxymethylcellulase B Cellulase 5B Cellulase B |
| Gene Name | eglB cel5B AKAW_03613 |
| Organism | Aspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus awamori var. kawachi) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus kawachii (White koji mold) (Aspergillus awamori var. kawachi) Aspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus awamori var. kawachi) |
| Enzyme Sequence | MKFQSTLLLAAAAGSALAVPHGPGHKKRASVFEWFGSNESGAEFGTNIPGVWGTDYIFPDPSAISTLIDKGMNFFRVQFMMERLLPDSMTGSYDEEYLANLTTVIKAVTDGGAHALVDPHNYGRYNGEIISSTSDFQTFWENLAGQYKDNDLVMFDTNNEYHDMDQDLVLNLNQAAINGIRAAGATSQYIFVEGNSWTGAWTWVDVNDNMKNLTDPEDKIVYEMHQYLDSDGSGTSETCVSETIGKERVTEATQWLKDNKKVGFIGEYAGGSNDVCRSAVSGMLEYMANNTDVWKGASWWAAGPWWGDYIFSMEPPDGTAYTGMLDILEAYL |
| Enzyme Length | 332 |
| Uniprot Accession Number | Q96WQ8 |
| Absorption | |
| Active Site | ACT_SITE 160; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 267; /note=Nucleophile; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | FUNCTION: Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Glycosylation (4); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cellulose degradation;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 36,733 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |