Detail Information for IndEnz0011000240
IED ID IndEnz0011000240
Enzyme Type ID glucanase000240
Protein Name Endoglucanase 5
EC 3.2.1.4
Endo-1,4-beta glucanase 5
Gene Name At1g48930 F27J15.28 F27K7.5
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MRKFGGSLFGVSLLLSVLLAAATAAAEYYNYGSALDKTFLFFEAQRSGKLPAAQRVKWRGPSGLKDGLAQGVSLEGGYYDAGDHVKFGLPMAFAVTMLSWAAVDNRKELSSSNQMQQTLWSIRWGTDYFIKAHPQPNVLWGQVGDGESDHYCWERPEDMTTSRTAYKLDPYHPGSDLAGETAAALAAASLAFKPFNSSYSALLLSHAKELFSFADKYRGLYTNSIPNAKAFYMSSGYSDELLWAAAWLHRATGDQYYLKYAMDNSGYMGGTGWGVKEFSWDNKYAGVQILLSKILLEGKGGIYTSTLKQYQTKADYFACACLKKNGGYNIQTTPGGLMYVREWNNLQYASAAAYLLAVYSDYLSAANAKLNCPDGLVQPQGLLDFARSQADYILGKNRQGMSYVVGYGPKYPIRVHHRGSSIPSIFAQRSSVSCVQGFDSWYRRSQGDPNVIYGALVGGPDENDNYSDDRSNYEQSEPTLSGTAPLVGLFAKLYGGSLGSYGGGSYKPYETTKPAASSYKATPTTYSPKQSGAQIEFLHSITSNWIAGNTRYYRHKVIIKNNSQKPISDLKLKIEDLSGPIWGLNPTGQKYTYQLPQWQKTLRAGQAYDFVYVQGGPQAKVSVLSYN
Enzyme Length 627
Uniprot Accession Number Q9M995
Absorption
Active Site ACT_SITE 83; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10140; ACT_SITE 416; /evidence=ECO:0000255|PROSITE-ProRule:PRU10059; ACT_SITE 468; /evidence=ECO:0000255|PROSITE-ProRule:PRU10059; ACT_SITE 477; /evidence=ECO:0000255|PROSITE-ProRule:PRU10059
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
DNA Binding
EC Number 3.2.1.4
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (4); Chain (1); Glycosylation (3); Signal peptide (1)
Keywords Carbohydrate metabolism;Cell wall biogenesis/degradation;Cellulose degradation;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal
Interact With Q9SZU7
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..26; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 16258017; 16489130; 16553896; 17408486; 17687051; 22253603; 23173056; 24844661;
Motif
Gene Encoded By
Mass 69,080
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda