IED ID | IndEnz0011000256 |
Enzyme Type ID | glucanase000256 |
Protein Name |
Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GGIB50 EC 3.2.1.39 1- 3 -beta-glucan endohydrolase 1- 3 -beta-glucanase Beta-1,3-endoglucanase, basic Glucanase GLA |
Gene Name | |
Organism | Nicotiana tabacum (Common tobacco) |
Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Nicotianoideae Nicotianeae Nicotiana Nicotiana tabacum (Common tobacco) |
Enzyme Sequence | MSTSHKHNTPQMAAITLLGLLLVASSIDIAGAQSIGVCYGMLGNNLPNHWEVIQLYKSRNIGRLRLYDPNHGALQALKGSNIEVMLGLPNSDVKHIASGMEHARWWVQKNVKDFWPDVKIKYIAVGNEISPVTGTSYLTSFLTPAMVNIYKAIGEAGLGNNIKVSTSVDMTLIGNSYPPSQGSFRNDARWFVDAIVGFLRDTRAPLLVNIYPYFSYSGNPGQISLPYSLFTAPNVVVQDGSRQYRNLFDAMLDSVYAALERSGGASVGIVVSESGWPSAGAFGATYDNAATYLRNLIQHAKEGSPRKPGPIETYIFAMFDENNKNPELEKHFGLFSPNKQPKYNINFGVSGGVWDSSVETNATASLVSEM |
Enzyme Length | 370 |
Uniprot Accession Number | P23546 |
Absorption | |
Active Site | ACT_SITE 128; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O22317; ACT_SITE 273; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O22317 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.; EC=3.2.1.39; |
DNA Binding | |
EC Number | 3.2.1.39 |
Enzyme Function | FUNCTION: Implicated in the defense of plants against pathogens. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (2); Chain (1); Erroneous initiation (1); Glycosylation (1); Modified residue (1); Propeptide (1); Sequence conflict (2); Signal peptide (1) |
Keywords | Direct protein sequencing;Glycoprotein;Glycosidase;Hydrolase;Plant defense;Pyrrolidone carboxylic acid;Reference proteome;Signal;Vacuole |
Interact With | |
Induction | INDUCTION: By ethylene and salicylic acid. |
Subcellular Location | SUBCELLULAR LOCATION: Vacuole. |
Modified Residue | MOD_RES 33; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000250|UniProtKB:P15797 |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..32 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 40,395 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |