Detail Information for IndEnz0011000257
IED ID IndEnz0011000257
Enzyme Type ID glucanase000257
Protein Name Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GLB
EC 3.2.1.39
1-
3
-beta-glucan endohydrolase
1-
3
-beta-glucanase
Beta-1,3-endoglucanase, basic
Glucanase GLB
Gene Name
Organism Nicotiana tabacum (Common tobacco)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Nicotianoideae Nicotianeae Nicotiana Nicotiana tabacum (Common tobacco)
Enzyme Sequence MSTSDKHNTPQMAAITLLGLLLVASTIEIAGAQSIGVCYGMLGNNLPNHWEVIQLYKSRNIGRLRLYDPNHGALQALKGSNIEVMLGLPNSDVKHIASGMEHARWWVQKNVKDFWPDVKIKYIAVGNEISPVTGTSYLTSFLTPAMVNIYKAIGEAGLGNNIKVSTSVDMTLIGNSYPPSQGSFRNDARWFTDPIVGFLRDTRAPLLVNIYPYFSYSGNPGQISLPYSLFTAPNVVVQDGSRQYRNLFDAMLDSVYAALERSGGASVGIVVSESGWPSAGAFGATYDNAATYLRNLIQHAKEGSPRKPGPIETYIFAMFDENNKNPELEKHFGLFSPNKQPKYNLNFGVSGGVWDSSVETNATASLISEM
Enzyme Length 370
Uniprot Accession Number P27666
Absorption
Active Site ACT_SITE 128; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O22317; ACT_SITE 273; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O22317
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.; EC=3.2.1.39;
DNA Binding
EC Number 3.2.1.39
Enzyme Function FUNCTION: Implicated in the defense of plants against pathogens.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Glycosylation (1); Modified residue (1); Propeptide (1); Signal peptide (1)
Keywords Glycoprotein;Glycosidase;Hydrolase;Plant defense;Pyrrolidone carboxylic acid;Reference proteome;Signal;Vacuole
Interact With
Induction INDUCTION: By ethylene and salicylic acid.
Subcellular Location SUBCELLULAR LOCATION: Vacuole.
Modified Residue MOD_RES 33; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000250|UniProtKB:P15797
Post Translational Modification
Signal Peptide SIGNAL 1..32
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 12411500;
Motif
Gene Encoded By
Mass 40,443
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda