Detail Information for IndEnz0011000260
IED ID IndEnz0011000260
Enzyme Type ID glucanase000260
Protein Name Manganese dependent endoglucanase Eg5A
EC 3.2.1.4
Carboxymethyl-cellulase 5A
CMCase 5A
Cellulase 5A
Endo-1,4-beta-glucanase Eg5A
Gene Name Eg5A cel5A
Organism Phanerodontia chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Agaricomycetes Agaricomycetes incertae sedis Polyporales Phanerochaetaceae Phanerodontia Phanerodontia chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Enzyme Sequence MLKYASIALALATLGVAQQQQWGQCGGIGWTGATTCVAGSVCSVLNPYYSQCIPGAATVTSSSAPSTPTPPAGALPRLGGVNTAGYDFSVATDGSFTGTGVSPPVSQFSHFSSQGANLYRIPFAWQLMTPTLGGTISQSFLSRYDQTVQAALNSGPNVFVIIDLHNYARWNGGIIAQGGPTDAQFQSIWTQLAQKYGSNQRVIFGIMNEPHDIPSISTWVNSVQGAVNAIRAAGATNYLLLPGSSWSSAQAFPTEAGPLLVKVTDPLGGTSKLIFDVHKYLDSDNSGTHPDCTTDNVQVLQTLVQFLQANGNRQAILSETGGGNTSSCESLLANELAYVKSAYPTLAGFSVWAAGAFDTTYVLTVTPNADGSDQPLWVDAVKPNLP
Enzyme Length 386
Uniprot Accession Number Q66NB7
Absorption
Active Site ACT_SITE 209; /note=Proton donor; /evidence=ECO:0000305|PubMed:26173955; ACT_SITE 319; /note=Nucleophile; /evidence=ECO:0000305|PubMed:26173955
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; Evidence={ECO:0000269|PubMed:26173955};
DNA Binding
EC Number 3.2.1.4
Enzyme Function FUNCTION: Secreted manganese dependent endoglucanase that acts by cleaving the beta-1,4-glucose linkage (PubMed:26173955). Exhibits high activity toward carboxymethyl-cellulose (CMC), barley glucan, and glucomannan (PubMed:26173955). Displays low activity on larminarin and xyloglucan but does not hydrolyze hemicellulose substrates such as birchwood xylan, arabinoxylan, and arabinan (PubMed:26173955). {ECO:0000269|PubMed:26173955}.
Temperature Dependency BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 50-60 degrees Celsius. {ECO:0000269|PubMed:26173955};
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4.5-6.0. {ECO:0000269|PubMed:26173955};
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Domain (1); Glycosylation (1); Signal peptide (1)
Keywords Carbohydrate metabolism;Cellulose degradation;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15888348}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..17; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 40,412
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=3.7 uM for carboxymethyl-cellulose (CMC) {ECO:0000269|PubMed:26173955}; Vmax=208 umol/min/mg enzyme toward carboxymethyl-cellulose (CMC) {ECO:0000269|PubMed:26173955};
Metal Binding
Rhea ID
Cross Reference Brenda 3.2.1.4;