IED ID | IndEnz0011000263 |
Enzyme Type ID | glucanase000263 |
Protein Name |
Endoglucanase EC 3.2.1.4 Cellulase Endo-1,4-beta-glucanase Spore germination protein 270-6 |
Gene Name | celA DDB_G0271134 |
Organism | Dictyostelium discoideum (Slime mold) |
Taxonomic Lineage | cellular organisms Eukaryota Amoebozoa Evosea Eumycetozoa Dictyostelia (dictyostelid cellular slime molds) Dictyosteliales Dictyosteliaceae Dictyostelium Dictyostelium discoideum (Slime mold) |
Enzyme Sequence | MKILKNCILLIIFGLLSTQLINADTDYCSLLENALMFYKMNRAGRLPDNDIPWRGNSALNDASPNSAKDANGDGNLSGGYFDAGDGVKFGLPMAYSMTMLGWSFIEYESNIAQCGLTSLYLDTIKYGTDWLIAAHTADNEFAGQVGDGNVDHSWWGPPEDMTMARPTYMLTTEAPGTEIAMEAASALAAASIAFKSSNPTYAATCLAHAKTLHNFGYTYRGVYSDSITNAQAFYNSWSGYKDDLVWGSIWLYKATQDSDYLTKAVADYASGGVGGMAQGNSHDWDNKAPGCCLLLSKLVPTTSTYKTDFEGWLNYWLPGGGVTYTPGGLAWIRQWGPARYAATAAFLGSLAGTEKGTDFTQKQVDYLIGNNPNQQSFVVGMGPNYPINPHHRAAHHSTTNDINNPVNNLYLLKGALVGGPGSNDEYTDDRTDYISNEVATDYNAGFVGALASLVNPSSTSVPTTTPTVTETPTETPTETPTETPTETPTETPTETPTETPTETPTETPTETPTETPTETPTETPTETPTETPTETPTETVTPTPTVTPTETPSSGESLSIYKSGLKNDFQDWSWGEHSLTDTTNVESGETNSISFTPKAYGAVFLGCFECIDTDTYNNIEFDINGGSSGAQLLRITVVKNSKSVGSKLITDLNGGTPIEANSWTKIKASFIDDFKVSGKVDGIWIQDIKGDTQSTVYISNIIATA |
Enzyme Length | 705 |
Uniprot Accession Number | P22699 |
Absorption | |
Active Site | ACT_SITE 85; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10140; ACT_SITE 390; /evidence=ECO:0000255|PROSITE-ProRule:PRU10059; ACT_SITE 428; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060; ACT_SITE 437; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
DNA Binding | |
EC Number | 3.2.1.4 |
Enzyme Function | FUNCTION: May digest the spore cell wall during germination, to release the enclosed amoeba. {ECO:0000269|PubMed:1869562}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (4); Chain (1); Glycosylation (1); Region (2); Signal peptide (1) |
Keywords | Carbohydrate metabolism;Cellulose degradation;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Repeat;Signal;Sporulation |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | 1447151; 15170254; 15470238; 7659526; 9334183; |
Motif | |
Gene Encoded By | |
Mass | 75,911 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |