Detail Information for IndEnz0011000268
IED ID IndEnz0011000268
Enzyme Type ID glucanase000268
Protein Name Probable endo-beta-1,4-glucanase D
Endoglucanase D
EC 3.2.1.4
Carboxymethylcellulase D
Cellulase D
Gene Name eglD AFUB_080950
Organism Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) (Aspergillus fumigatus)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Fumigati Neosartorya fumigata (Aspergillus fumigatus) Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) (Aspergillus fumigatus)
Enzyme Sequence MKSTFGLLALAAAAKLVSAHATVHAVWINDVDQGAGNSADGYIRSPPNNSPITDVTSTDMTCNVNGKNPVAKTLSVKAGDKVTFEWHHDTRSDSDDIIASSHMGPVMVYMAPTEKGTAGNGWVKIAEEGYSNGKWAVANLIANRGKHSITVPDVPAGEYLLRPEIIALHEGNRQGGAQFYMECVQVKVTSAGTKTLPAGVSIPGAYKATDPGVLFDMYNSFTSYPIPGPAVWDGSSSGSSGSSGSSPATTTAPAVSVTAVPTKEAPVDTSATPTTFVTATKPATTAAPAAPSASSGSNSGSDSCNSGSASGSVKIYGQCGGQNYSGPTSCEAGLICKEWNPYYHQCVSA
Enzyme Length 349
Uniprot Accession Number B0Y9G4
Absorption
Active Site ACT_SITE 164; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 210; /note=Nucleophile; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
DNA Binding
EC Number 3.2.1.4
Enzyme Function FUNCTION: Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Disulfide bond (2); Domain (1); Glycosylation (1); Region (3); Signal peptide (1)
Keywords Carbohydrate metabolism;Cellulose degradation;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..19; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 35,763
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda