Detail Information for IndEnz0011000279
IED ID IndEnz0011000279
Enzyme Type ID glucanase000279
Protein Name Probable glucan endo-1,6-beta-glucosidase B
EC 3.2.1.75
Beta-1,6-glucanase B
Endo-1,6-beta-D-glucanase B
Endo-1,6-beta-glucanase B
Gene Name exgB AFLA_045690
Organism Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus flavus Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167)
Enzyme Sequence MKVTRLAVLNTLATLTVAWLPTTDKTITSSNGTDLFKASHGKIRGVNLGSQFVFEPWIATKAWSELGCEGQESEFDCVMKLGQDAANKAFAKHWDSWITKEDIKEIRSYGLNTIRIPVGYWMNEDLIYHDSEYFPHGGFAYLEKLCGWASDAGLYIIIDLHGAPGAQVAKNAFTGQFADTPGFYVDFQYQRALEFLEWMTIKVHTLHNFRNVGMLEVVNEPVQNPQVTTTLRSNYYPNAFHSIRKVEGALSIDRKDYLHIQMMDGAWGAGDPHEHLTDDYYAAYDNHRYLKWDPRVEVSKDSYIKTSCNDNVATNWPAIIGEWSLGVPDNVQETADWKPYSNLDFYQKWFAAQVQNYEQHQGWIFWTWKTQLDEYRWSYRGTYLSGFWQTSS
Enzyme Length 392
Uniprot Accession Number B8NBJ4
Absorption
Active Site ACT_SITE 220; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 322; /note=Nucleophile; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Random hydrolysis of (1->6)-linkages in (1->6)-beta-D-glucans.; EC=3.2.1.75;
DNA Binding
EC Number 3.2.1.75
Enzyme Function FUNCTION: Beta-glucanases participate in the metabolism of beta-glucan, the main structural component of the cell wall. Acts on lutean, pustulan and 1,6-oligo-beta-D-glucosides (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Glycosylation (1); Signal peptide (1)
Keywords Carbohydrate metabolism;Cell wall biogenesis/degradation;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..18; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 45,214
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda