| IED ID | IndEnz0011000289 |
| Enzyme Type ID | glucanase000289 |
| Protein Name |
Endoglucanase 3 EC 3.2.1.4 Cellulase 5 AtCEL5 Endo-1,4-beta glucanase 3 |
| Gene Name | CEL5 At1g22880 F19G10.16 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MASPFFFVFLLSALSLENTYASPNYREALSKSLLFFQGQRSGRLPSDQQLSWRSSSGLSDGSSAHVDLTGGYYDAGDNVKFNFPMAFTTTMLSWSSLEYGKKMGPELQNSRVAIRWATDYLLKCARATPGKLYVGVGDPNGDHKCWERPEDMDTPRTVYSVSPSNPGSDVAAETAAALAASSMVFRKVDPKYSRLLLATAKKVMQFAIQYRGAYSNSLSSSVCPFYCSYSGYKDELLWGAAWLHRATNDPYYTNFIKSLGGGDQPDIFSWDNKYAGAYVLLSRRAVLNKDNNFELYKQAAENFMCKILPNSPSSSTKYTKGGLMYKLPQSNLQYVTSITFLLTTYAKYMKSTKQTFNCGNSLIVPNALINLSKRQVDYVLGVNPMKMSYMVGFSSNFPKRIHHRGSSLPSRAVRSNSLGCNGGFQSFRTQNPNPNILTGAIVGGPNQNDEYPDQRDDYTRSEPATYINAAFVGPLAYFAASRSP |
| Enzyme Length | 484 |
| Uniprot Accession Number | Q2V4L8 |
| Absorption | |
| Active Site | ACT_SITE 77; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10140; ACT_SITE 402; /evidence=ECO:0000255|PROSITE-ProRule:PRU10059; ACT_SITE 453; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060; ACT_SITE 462; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | FUNCTION: May be involved in the sloughing (cell-cell separation) of the root cap cells from root tip. {ECO:0000269|PubMed:15604746}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (4); Alternative sequence (1); Chain (1); Glycosylation (1); Signal peptide (1) |
| Keywords | Alternative splicing;Carbohydrate metabolism;Cell wall biogenesis/degradation;Cellulose degradation;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | INDUCTION: Down-regulated by auxin (IAA) and abscisic acid (ABA). {ECO:0000269|PubMed:15604746}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14576160; 15047898; 16287169; 16776300; 17317660; 17687051; 18577427; 27221617; 27247031; |
| Motif | |
| Gene Encoded By | |
| Mass | 53,662 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |