| IED ID | IndEnz0011000298 |
| Enzyme Type ID | glucanase000298 |
| Protein Name |
Endoglucanase A EC 3.2.1.4 Endo-1,4-beta-glucanase A |
| Gene Name | eglA |
| Organism | Bacillus pumilus (Bacillus mesentericus) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus pumilus (Bacillus mesentericus) |
| Enzyme Sequence | MLIFETYLILFKTVQITKRRIERRRLRLLNQCFTKKEGVSNREMASYNYVEVLQKSMLFYEAQRSGRLPESNRLNWRGDSGLKDGKDVGHDLTGGWYDAGDHVKFGLPMAYSAAVLAWTVYEYREAYEEAELLDEILDQIKWATDYFLKAHTGPNEFWAQVGDGNADHAWWGPAEVMPMNRPAFKIDEHCPGTEVAAQTAAALAAGSIIFKETDASYAAKLLTHAKQLYAFADRYRGKYTDCVTNAQPFYNSWSGYVDELIWGGIWLYLATNEETYLNKALKAVEEWPQDWDYTFTMSWDNTFFASQILLARITKENRFIESTERNLDYWTTGLVQNGKVERITYTPGGLAWLDQWGSLRYAANAAFLAFVYADWVSDQEKKNRYQSFAIKQTHYMLGDNPLNRSYVVGFGQNSPKHPHHRTAHGSWSNQLTNPPSHRHTLYGALVGGPNAQDQYDDDISDYISNEVATDYNAAFTGNIAKMVQLFGEGQSKLPNFPPKEQVEDEFFVEAAVMHNDTTSTQVKAVLYNRSGWPARSSQTLSFRYYVNLSEVFAKGFTEKDIQVTAAYNEGASLSPLKVYDASSRVYFAEIDFTGVVISPRGESEHKKEIQFRLSAPNGSNIWDASNDYSYQGLTSNMQKTTKIPVFEDGVLVFGTLPDK |
| Enzyme Length | 659 |
| Uniprot Accession Number | Q5YLG1 |
| Absorption | |
| Active Site | ACT_SITE 101; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10140; ACT_SITE 419; /evidence=ECO:0000255|PROSITE-ProRule:PRU10059; ACT_SITE 457; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060; ACT_SITE 466; /evidence=ECO:0000255|PROSITE-ProRule:PRU10060 |
| Activity Regulation | ACTIVITY REGULATION: Strongly inhibited by ZnCl(2) and by EDTA. {ECO:0000269|PubMed:15538558}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; Evidence={ECO:0000269|PubMed:15538558}; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | FUNCTION: Active on carboxymethyl cellulose and carboxymethyl cellulose-RBB but not avicel, xanthan gum, carboxymethyl-curdulan-RBB or carboxymethyl-xylan-RBB. {ECO:0000269|PubMed:15538558}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 60 degrees Celsius. Retains more than 90% activity after 24 hours at 50 degrees Celsius. {ECO:0000269|PubMed:15538558}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5-8. {ECO:0000269|PubMed:15538558}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (4); Chain (1); Domain (1); Region (2) |
| Keywords | Carbohydrate metabolism;Cellulose degradation;Glycosidase;Hydrolase;Polysaccharide degradation;Secreted |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15538558}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 74,985 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |