| IED ID | IndEnz0011000303 |
| Enzyme Type ID | glucanase000303 |
| Protein Name |
Endoglucanase A EC 3.2.1.4 Cellulase A Endo-1,4-beta-glucanase A |
| Gene Name | celA |
| Organism | Clostridium longisporum |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Clostridia Eubacteriales Clostridiaceae Clostridium Clostridium longisporum |
| Enzyme Sequence | MKRSLLKTCSIIAGATIIFSSLSISRNPLEVQAASMRSASEIVQEMGVGWNLGNTLDAKITNLSYNTSPISFETGWGNPVTTKAMIDKIKNAGFKTIRIPTTWGEHLDGNNKLNEEWVKRVKEVVDYCIADDLYVILNTHHEGNWVIPTYAKESSVTPKLKTLWTQISEAFKDYDDHLIFETLNEPRLEGTPYEWTGGTSESRDVVNKYNAAALESIRKTGGNNLSRAVMMPTYAASGSSTTMNDFKVPDDKNVIASVHAYSPYFFAMDTSSNSVNTWGSSYDKYSLDVELDSYLNTFKSKGVPVVIGEFGSINKNNTSSRAELAEYYVTAAQKRGIPCVWWDNNYAETNKGETFGLLNRSTLNWYFSDIKDALIRGYKNVHPEATEDDKPSTDVTNPDSGNTKPDSGNTNPGTETTTPTDNEKISITSKINDWGGAYQADFTLKNNTSSDINNWSFKIKKNDIVFTNYWDVKITEENGYYVVTPQAWKTTILANSSIVISIQGTGKVISNFEYKFD |
| Enzyme Length | 517 |
| Uniprot Accession Number | P54937 |
| Absorption | |
| Active Site | ACT_SITE 185; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 309; /note=Nucleophile; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | FUNCTION: Hydrolyzes barley beta-glucan, lichenan, carboxymethylcellulose and xylan. It shows preferential activity against the larger cellooligosaccharides (cellohexaose and cellopentaose); cellotetraose is the smallest substrate degraded completely. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 43 degrees Celsius.; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4.8.; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (9); Chain (1); Compositional bias (1); Domain (1); Helix (13); Region (2); Signal peptide (1); Turn (6) |
| Keywords | 3D-structure;Carbohydrate metabolism;Cellulose degradation;Direct protein sequencing;Glycosidase;Hydrolase;Polysaccharide degradation;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..25; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 6Q1I; |
| Mapped Pubmed ID | 33454012; |
| Motif | |
| Gene Encoded By | |
| Mass | 57,660 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |