| IED ID | IndEnz0011000304 |
| Enzyme Type ID | glucanase000304 |
| Protein Name |
Endoglucanase A EC 3.2.1.4 Cellulase Endo-1,4-beta-glucanase |
| Gene Name | endA |
| Organism | Fibrobacter succinogenes (Bacteroides succinogenes) |
| Taxonomic Lineage | cellular organisms Bacteria FCB group Fibrobacteres Fibrobacteria Fibrobacterales Fibrobacteraceae Fibrobacter Fibrobacter succinogenes (Bacteroides succinogenes) |
| Enzyme Sequence | MNCRKYLLSGLAVFGLAATSAVAALSTDDYVEAAWMTTRFFGAQRSGQGPNWILDGTSNPTSFTKDSYNGKDVSGGWFDCGDHVMYGQSQGYASYVLALAYAEFTEVSTTFILVTTPTTRKPTTTPMKSGKPNKVRDLLEELRYEADFWVKAAIDGNNFVTVKGDGNADHQKWVTAGAMSKLGSGEGGEPRCITGNANDGFTSGLAAAMLAVMARVDPDTANQAKYLKAAKTAYSYAKSHKGVTNSQGFYESSWWDGRWEDGPFLAELELYRTTGENSYKTAAIDRYDNLKFSLGEGTHFMYSNVVPLSAVMAEAVFEETPHGMRKEAIGVLDLIYEEKAKDKIFQNPNGMGSGKFPVRVPSGGAFLYALSDKFNNTNEHMEMIEKNVSYLLGDNGSKKSYVVGFSKNGANAPSRPHHRGYYANEKRWRRSRRCSESSRKEQALGRYDCWRLY |
| Enzyme Length | 453 |
| Uniprot Accession Number | P23665 |
| Absorption | |
| Active Site | ACT_SITE 82; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10140; ACT_SITE 417; /evidence=ECO:0000255|PROSITE-ProRule:PRU10059 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | FUNCTION: High levels of endoglucanase activity detected on acid-swollen cellulose, ball-milled cellulose, and carboxymethyl cellulose; moderate levels detected on filter paper, phosphoric acid-swollen cellulose, lichenan, and xylan. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 37-39 degrees Celsius.; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.0.; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Frameshift (1); Region (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cellulose degradation;Glycosidase;Hydrolase;Periplasm;Polysaccharide degradation;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Periplasm {ECO:0000269|PubMed:2014986}. Note=80% periplasmic, 16% extracellular upon overexpression in E.coli. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..26; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 50,042 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |