| IED ID | IndEnz0011000306 |
| Enzyme Type ID | glucanase000306 |
| Protein Name |
Probable endoglucanase EC 3.2.1.4 Cellulase Endo-1,4-beta-glucanase |
| Gene Name | cmcAX |
| Organism | Komagataeibacter hansenii (Gluconacetobacter hansenii) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Alphaproteobacteria Rhodospirillales Acetobacteraceae Komagataeibacter Komagataeibacter hansenii (Gluconacetobacter hansenii) |
| Enzyme Sequence | MSVMAAMGGAQVLSSTGAFADPAPDAVAQQWAIFRAKYLRPSGRVVDTGNGGESHSEGQGYGMLFAASAGDLASFQSMWMWARTNLQHTNDKLFSWRFLKGHQPPVPDKNNATDGDLLIALALGRAGKRFQRPDYIQDAMAIYGDVLNLMTMKAGPYVVLMPGAVGFTKKDSVILNLSYYVMPSLLQAFDLTADPRWRQVMEDGIRLVSAGRFGQWRLPPDWLAVNRATGALSIASGWPPRFSYDAIRVPLYFYWAHMLAPNVLADFTRFWNNFGANALPGWVDLTTGARSPYNAPPGYLAVAECTGLDSAGELPTLDHAPDYYSAALTLLVYIARAEETIK |
| Enzyme Length | 342 |
| Uniprot Accession Number | P37696 |
| Absorption | |
| Active Site | ACT_SITE 57; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 114; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10058 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | FUNCTION: Enzyme capable of hydrolyzing carboxy-methyl-cellulose (CMC). |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (13); Chain (1); Helix (13); Signal peptide (1); Turn (4) |
| Keywords | 3D-structure;Carbohydrate metabolism;Cellulose degradation;Direct protein sequencing;Glycosidase;Hydrolase;Polysaccharide degradation;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000269|PubMed:8300521 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 1WZZ; |
| Mapped Pubmed ID | 16804941; |
| Motif | |
| Gene Encoded By | |
| Mass | 37,443 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |