| IED ID | IndEnz0011000334 |
| Enzyme Type ID | glucanase000334 |
| Protein Name |
Endoglucanase D EC 3.2.1.4 Cellulase D Endo-1,4-beta-glucanase D |
| Gene Name | engD Clocel_3242 |
| Organism | Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Clostridia Eubacteriales Clostridiaceae Clostridium Clostridium cellulovorans Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B) |
| Enzyme Sequence | MIKHLLSRGKLLLFVSVMATSSIIAGGNAYGSTAFTGVRDVPAQQIVNEMKVGWNLGNTMDAIGGETNWGNPMTTHAMINKIKEAGFNTLRLPVTWDGHMGAAPEYTIDQTWMKRVEEIANYAFDNDMYVIINLHHENEWLKPFYANEAQVKAQLTKVWTQIANNFKKYGDHLIFETMNEPRPVGASNEWTGGSYENREVVNRYNLTAVNAIRATGGNNATRYIMVPTLAASAMSTTINDLVIPNNDSKVIVSLHMYSPYFFAMDINGTSSWGSDYDKSSLDSEFDAVYNKFVKNGRAVVIGEMGSINKNNTAARVTHAEYYAKSAKARGLTPIWWDNGYSVAGKAETFGIFNRSNLTWDAPEVMKAFIKGIGGSSTTTPTTPTTPTTPTTPTTPTTPTTPTTPTTPQSAVEVTYAITNSWGSGASVNVTIKNNGTTPINGWTLKWTMPINQTITNMWSASFVASGTTLSVTNAGYNGTIAANGGTQSFGFNINYSGVLSKPTGFTVNGTECTVK |
| Enzyme Length | 515 |
| Uniprot Accession Number | P28623 |
| Absorption | |
| Active Site | ACT_SITE 180; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 303; /note=Nucleophile; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | FUNCTION: Has endoglucanase activity on carboxymethyl-cellulose (CMC), cellobiosidase activity on p-nitrophenyl-cellobioside (p-NPC), and partial hydrolytic activity on crystalline cellulose (Avicel). |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (17); Chain (1); Compositional bias (1); Domain (1); Helix (15); Region (2); Sequence conflict (1); Signal peptide (1); Turn (10) |
| Keywords | 3D-structure;Carbohydrate metabolism;Cellulose degradation;Direct protein sequencing;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..31; /evidence=ECO:0000269|PubMed:1538700 |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 3NDY; 3NDZ; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 55,978 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |