| IED ID | IndEnz0011000336 |
| Enzyme Type ID | glucanase000336 |
| Protein Name |
Endoglucanase E EGE EC 3.2.1.4 Cellulase Endo-1,4-beta-glucanase E |
| Gene Name | celE |
| Organism | Ruminococcus flavefaciens |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Clostridia Eubacteriales Oscillospiraceae Ruminococcus Ruminococcus flavefaciens |
| Enzyme Sequence | MRKRITSMITVISLTLCGTIGTYPHNANASAKEAFEDISKRSECTKVFDNDNGTYTAYSNTAPIHYLNNDEWKEIDNTLIEDSDDYYRNKDNSFNIYIPKEYSLGKNIKNPVIMNYDTFSLSTSITDIKMPDRPEEETFSEGVYADINNEVYTTIDNSAIAEGMKAALKKSASMATYHSIVEDIDLDIAVHNASVSESIIINKLESLPETITYSISVEDAIIKKTEDNRLQLIKDNEAVLILSPFTISDSSENVNVMQVEYDLTESEEGYEVTLYPAETVNRMSSPVMPLSLGSEYSYDRPFSTVYNSQASLHPSIIIIT |
| Enzyme Length | 320 |
| Uniprot Accession Number | Q05622 |
| Absorption | |
| Active Site | ACT_SITE 150; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | FUNCTION: Hydrolyzes both carboxymethylcellulose and xylan. Probably has a role in hydrolyzing oligosaccharides derived from cellulose, which are transported across the cell wall. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cellulose degradation;Glycosidase;Hydrolase;Polysaccharide degradation;Secreted;Signal;Xylan degradation |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..29; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 35,938 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |