| IED ID | IndEnz0011000366 |
| Enzyme Type ID | glucanase000366 |
| Protein Name |
Endoglucanase EC 3.2.1.4 Carboxymethylcellulase CMCase Cellulase Endo-1,4-beta-glucanase |
| Gene Name | bcsZ wssD PFLU_0303 |
| Organism | Pseudomonas fluorescens (strain SBW25) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas fluorescens group (fluorescent pseudomonads) Pseudomonas fluorescens Pseudomonas fluorescens (strain SBW25) |
| Enzyme Sequence | MSPLKCMALAALGAVMFVGSAQAQTCDWPLWQNYAKRFVQDDGRVLNSSMKPTESSSEGQSYAMFFALVGNDRASFDKLWTWTKANMSGADIGQNLPGWLWGKKADNTWGVIDPNSASDADLWMAYALLEAARVWNAPQYRADAQLLLANVERNLIVRVPGLGKMLLPGPVGYVHAGGLWRFNPSYQVLAQLRRFHKERPNAGWNEVADSNAKMLADTASNPHGLAANWVGYRATSANTGLFVVDPFSDDLGSYDAIRTYMWAGMTAKGDPLAAPMLKSLGGMTRATAASATGYPPEKIHVLTGEVEKNNGYTPMGFSASTVAFFQARGETALAQLQKAKVDDALAKALAPSAPDTAQPIYYDYMLSLFSQGFADQKYRFEQDGTVKLSWEAACAVTR |
| Enzyme Length | 398 |
| Uniprot Accession Number | Q8RSY9 |
| Absorption | |
| Active Site | ACT_SITE 58; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 119; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10058 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | FUNCTION: Hydrolyzes carboxymethylcellulose. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan metabolism; bacterial cellulose biosynthesis. |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Erroneous initiation (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cellulose degradation;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 43,345 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |