| IED ID | IndEnz0011000375 |
| Enzyme Type ID | glucanase000375 |
| Protein Name |
Probable glucan endo-1,3-beta-glucosidase BG3 EC 3.2.1.39 Beta-1,3-glucanase 3 AtBG3 |
| Gene Name | BG3 At3g57240 F28O9.90 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MKMCNGSSFLASLPLLLLLLSFILASFFDTAVGQIGVCYGRNGNNLRPASEVVALYQQRNIRRMRLYDPNQETLNALRGSNIELVLDVPNPDLQRLASSQAEADTWVRNNVRNYANVTFRYISVGNEVQPSDQAASFVLPAMQNIERAVSSLGIKVSTAIDTRGISGFPPSSGTFTPEFRSFIAPVISFLSSKQSPLLVNNYPYFSYTGNMRDIRLDYTLFTAPSTVVNDGQNQYRNLFHAILDTVYASLEKAGGGSLEIVVSESGWPTAGGAATGVDNARTYVNNLIQTVKNGSPRRPGRATETYIFAMFDENSKQGPETEKFWGLFLPNLQPKYVVNFN |
| Enzyme Length | 341 |
| Uniprot Accession Number | F4J270 |
| Absorption | |
| Active Site | ACT_SITE 127; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O22317; ACT_SITE 264; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O22317 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.; EC=3.2.1.39; Evidence={ECO:0000305}; |
| DNA Binding | |
| EC Number | 3.2.1.39 |
| Enzyme Function | FUNCTION: May play a role in plant defense against pathogens. {ECO:0000305|PubMed:23656331}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Erroneous gene model prediction (2); Glycosylation (1); Sequence conflict (1); Signal peptide (1) |
| Keywords | Endoplasmic reticulum;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | INDUCTION: By infection with a virulent strain of P.syringae pv. maculicola (PubMed:1824335). Induced by infection with the biotrophic powdery mildew pathogens Golovinomyces cichoracearum and Blumeria graminis (PubMed:16473969). Induced by infection with the turnip vein clearing virus (TVCV) and cucumber mosaic virus (CMV) (PubMed:23656331). {ECO:0000269|PubMed:16473969, ECO:0000269|PubMed:1824335, ECO:0000269|PubMed:23656331}. |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum {ECO:0000305|PubMed:23656331}. Secreted {ECO:0000250|UniProtKB:P33157}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..25; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14742872; 14966217; 15047898; 15282545; 15593128; 15763661; 16500996; 16766691; 18538804; 18650403; 28627464; 29777502; |
| Motif | |
| Gene Encoded By | |
| Mass | 37,632 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |