| IED ID | IndEnz0011000381 |
| Enzyme Type ID | glucanase000381 |
| Protein Name |
Probable endo-1,3 4 -beta-glucanase AFUB_029980 EC 3.2.1.6 Mixed-linked glucanase AFUB_029980 |
| Gene Name | AFUB_029980 |
| Organism | Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) (Aspergillus fumigatus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Fumigati Neosartorya fumigata (Aspergillus fumigatus) Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) (Aspergillus fumigatus) |
| Enzyme Sequence | MAPSSLLLSVGSLITSSLVSATALEARQSQTYQLAESWQGESFINDWNFFDGADPTNGYVTYVNQSFAKQSGLVKVTESGSFYMGVDYESTLNPNGAGRESVRIESKNYYTEGLYVIDIEHMPGSICGTWPAFWSVGKNWPNDGEIDIIEGVNLQKANKIVLHTSGSCDVSGSNDMTGTLSSSECGEASGTVGCVVKGTNGSSGDPFNESGGGVYAMEWTDTFIKIWFFPRSQIPASLASGNPDTSSFGTPMAHLQGSCDFAERFKAQKLIIDTTFCGDWAGNVFAESTCPMSDPSSPMQSCVNYVAQNPAAFKEAYWEINSIKIYQYGVSAASSAAVSQATASKVEGTRVSAQAANTATPTVPAPVETTTVPQPAQTNTVATSAADHATPSSAETTTVPAATGAPSVSATEGGDSELESTSTVYVTSTTTICPVAESSSAAAAGGKEDAPSNGTSGAEVAATSVAAAAPAAATSGHPGADAIANSAAATSTDAQSESATSRLTAGALSEIPTAPPEPVSQAVSTGSFDDSDTAQGDSEEQGSIASASVAPSTIPVPASSSAAALGGSSIASSFASSRLIPRPTGSSTAASATAIATWSPTAGESASGTAKESATLTTPSEVFFTPGLSNGANRMSVGLSGLIGVMFIAALA |
| Enzyme Length | 652 |
| Uniprot Accession Number | B0XTU6 |
| Absorption | |
| Active Site | ACT_SITE 145; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 150; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.; EC=3.2.1.6; |
| DNA Binding | |
| EC Number | 3.2.1.6 |
| Enzyme Function | FUNCTION: Mixed-linked glucanase involved in the degradation of complex natural cellulosic substrates. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (1); Domain (1); Glycosylation (4); Lipidation (1); Propeptide (1); Region (2); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cell membrane;Cellulose degradation;GPI-anchor;Glycoprotein;Glycosidase;Hydrolase;Lipoprotein;Membrane;Polysaccharide degradation;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Lipid-anchor, GPI-anchor {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 65,993 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |