| IED ID | IndEnz0011000385 |
| Enzyme Type ID | glucanase000385 |
| Protein Name |
Endoglucanase 3 EC 3.2.1.4 Cellulase 3 Endo-1,4-beta-glucanase 3 EG3 |
| Gene Name | cel-3 Fisuc_2230 FSU_2772 |
| Organism | Fibrobacter succinogenes (strain ATCC 19169 / S85) |
| Taxonomic Lineage | cellular organisms Bacteria FCB group Fibrobacteres Fibrobacteria Fibrobacterales Fibrobacteraceae Fibrobacter Fibrobacter succinogenes (Bacteroides succinogenes) Fibrobacter succinogenes subsp. succinogenes Fibrobacter succinogenes (strain ATCC 19169 / S85) |
| Enzyme Sequence | MQLKNFYPKMSVLGIATVMALTACGDENTQALFANNPVPGAENQVPVSSSDMSPTSSDAVIDPTSSSAAVVDPSTLPAEGPITMPEGLGTLVDDFEDGDNLSKIGDYWYTYNDNDNGGASIITTPLNEEENIIPGRVNNGSNYALQVNYTLDRGDYEYDPYVGWGVQVAPDEANGHFGGLTYWYKGGAHEVHIEITDVEDYDVHLAKFPASRTWKQAVVRFKDLVQGGWGKEIPFDAKHIMAISFQAKGNKSKLVTDSLFIDNIYLQDSSEVEKDQPDMEIKDPVIPVVEFTEAEITVTNPLQEKAMKYLNKGVNFTNWLENADGKFKSFELGESDVKILADNGFKSLRLPIDLDLYATNRDAFIAGTDTELKFDDDTLFLVLDSFVEWTAKYNMSFVIDYHEYDNSYNTTSAKDPNYIKMMAETWKHVAAHYAESPREDLFFELLNEPDMSDGKVTAATWTTAAQAMIDAIRTVDTKHTILFGDAQWYSITLLAKRTPFTDDNIIYVIHTYEPFAFTHQGGSWTDYATIHDIPFPYDPAKWSTVSGDFGVNKSTKSYVKTNIKNYYKTGSKEAILEQILKAKKWAATNNVPVIINEFGALNLRSTAESRLNYLTAMREICDTLQIPWTHWGYTGNFSVIENGKLIEGLDKALGVGSK |
| Enzyme Length | 658 |
| Uniprot Accession Number | P14250 |
| Absorption | |
| Active Site | ACT_SITE 448; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 597; /note=Nucleophile; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | FUNCTION: Exhibits both endoglucanase and cellobiosidase activities. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (1); Domain (1); Lipidation (2); Propeptide (1); Region (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cellulose degradation;Direct protein sequencing;Glycosidase;Hydrolase;Lipoprotein;Membrane;Palmitate;Polysaccharide degradation;Reference proteome;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}. |
| Modified Residue | |
| Post Translational Modification | PTM: May be a lipoprotein and may be glycosylated. |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255|PROSITE-ProRule:PRU00303 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 73,424 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |