| IED ID | IndEnz0011000397 |
| Enzyme Type ID | glucanase000397 |
| Protein Name |
Glucan endo-1,3-beta-glucosidase 12 EC 3.2.1.39 1- 3 -beta-glucan endohydrolase 12 1- 3 -beta-glucanase 12 Beta-1,3-endoglucanase 12 Beta-1,3-glucanase 12 |
| Gene Name | At4g29360 F17A13.180 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MGQRLNLVFWIFVSILAFLNFGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLKPMLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLYMIAPATDGFNRTMAGNITGNITAIDSPLASPSSTNEAFRQMVVAVSVLLPCFVVCSSIW |
| Enzyme Length | 534 |
| Uniprot Accession Number | Q8VYE5 |
| Absorption | |
| Active Site | ACT_SITE 120; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O22317; ACT_SITE 264; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O22317 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.; EC=3.2.1.39; |
| DNA Binding | |
| EC Number | 3.2.1.39 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (1); Chain (1); Disulfide bond (1); Erroneous gene model prediction (1); Glycosylation (7); Lipidation (1); Propeptide (1); Region (1); Signal peptide (1) |
| Keywords | Alternative splicing;Cell membrane;Cell wall;Cell wall biogenesis/degradation;Disulfide bond;GPI-anchor;Glycoprotein;Glycosidase;Hydrolase;Lipoprotein;Membrane;Plant defense;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000305}. Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. |
| Modified Residue | |
| Post Translational Modification | PTM: Contains two additional disulfide bonds. {ECO:0000250}. |
| Signal Peptide | SIGNAL 1..24; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12068095; 12805588; 14517339; 15937229; 16776300; 29141655; |
| Motif | |
| Gene Encoded By | |
| Mass | 57,659 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |