Detail Information for IndEnz0011000400
IED ID IndEnz0011000400
Enzyme Type ID glucanase000400
Protein Name Glucan endo-1,3-beta-glucosidase
EC 3.2.1.39
1-
3
-beta-glucan endohydrolase
1-
3
-beta-glucanase
Beta-1,3-endoglucanase
Gene Name SP41A
Organism Nicotiana tabacum (Common tobacco)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Nicotianoideae Nicotianeae Nicotiana Nicotiana tabacum (Common tobacco)
Enzyme Sequence MALWYLFNKRSLGAAVLILVGLLMCNIQMTGAQSNIGVCYGKIANNLPSEQDVINLYKANGIRKMRIYNSDTNIFKSLNGSNIEIILDVPNQDLEALANSSIANGWVQDNIRSHFPYVKFKYISIGNEVSPSNNGQYSQFLLHAMENVYNALAAAGLQDKIKVTTATYSGLLANTYPPKDSIFREEFKSFINPIIEFLARNNLPLLANIYPYFGHIYNTVDVPLSYALFNQQGTNSTGYQNLFDALLDSIYFAVEKAGGPNVEIIVSESGWPSEGNSAATIENAQTYYRNLVNHVKGGAGTPKKPGRIVETYLFAMFDENEKNGEVTEKHFGLFYPNRTAKYQLNFMYSDT
Enzyme Length 351
Uniprot Accession Number P23432
Absorption
Active Site ACT_SITE 128; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O22317; ACT_SITE 268; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O22317
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.; EC=3.2.1.39;
DNA Binding
EC Number 3.2.1.39
Enzyme Function FUNCTION: Implicated in the defense of plants against pathogens.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Glycosylation (4); Modified residue (1); Signal peptide (1)
Keywords Direct protein sequencing;Extracellular matrix;Glycoprotein;Glycosidase;Hydrolase;Hypersensitive response;Pathogenesis-related protein;Plant defense;Pyrrolidone carboxylic acid;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix {ECO:0000269|PubMed:2120041, ECO:0000269|PubMed:8555460}. Note=Stylar secretory matrix.
Modified Residue MOD_RES 33; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000269|PubMed:2120041
Post Translational Modification PTM: Glycosylated. {ECO:0000269|PubMed:2120041}.; PTM: The N-terminus is blocked. {ECO:0000269|PubMed:2120041}.
Signal Peptide SIGNAL 1..32
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 39,186
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda