| IED ID | IndEnz0011000400 |
| Enzyme Type ID | glucanase000400 |
| Protein Name |
Glucan endo-1,3-beta-glucosidase EC 3.2.1.39 1- 3 -beta-glucan endohydrolase 1- 3 -beta-glucanase Beta-1,3-endoglucanase |
| Gene Name | SP41A |
| Organism | Nicotiana tabacum (Common tobacco) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Nicotianoideae Nicotianeae Nicotiana Nicotiana tabacum (Common tobacco) |
| Enzyme Sequence | MALWYLFNKRSLGAAVLILVGLLMCNIQMTGAQSNIGVCYGKIANNLPSEQDVINLYKANGIRKMRIYNSDTNIFKSLNGSNIEIILDVPNQDLEALANSSIANGWVQDNIRSHFPYVKFKYISIGNEVSPSNNGQYSQFLLHAMENVYNALAAAGLQDKIKVTTATYSGLLANTYPPKDSIFREEFKSFINPIIEFLARNNLPLLANIYPYFGHIYNTVDVPLSYALFNQQGTNSTGYQNLFDALLDSIYFAVEKAGGPNVEIIVSESGWPSEGNSAATIENAQTYYRNLVNHVKGGAGTPKKPGRIVETYLFAMFDENEKNGEVTEKHFGLFYPNRTAKYQLNFMYSDT |
| Enzyme Length | 351 |
| Uniprot Accession Number | P23432 |
| Absorption | |
| Active Site | ACT_SITE 128; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O22317; ACT_SITE 268; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O22317 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.; EC=3.2.1.39; |
| DNA Binding | |
| EC Number | 3.2.1.39 |
| Enzyme Function | FUNCTION: Implicated in the defense of plants against pathogens. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Glycosylation (4); Modified residue (1); Signal peptide (1) |
| Keywords | Direct protein sequencing;Extracellular matrix;Glycoprotein;Glycosidase;Hydrolase;Hypersensitive response;Pathogenesis-related protein;Plant defense;Pyrrolidone carboxylic acid;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix {ECO:0000269|PubMed:2120041, ECO:0000269|PubMed:8555460}. Note=Stylar secretory matrix. |
| Modified Residue | MOD_RES 33; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000269|PubMed:2120041 |
| Post Translational Modification | PTM: Glycosylated. {ECO:0000269|PubMed:2120041}.; PTM: The N-terminus is blocked. {ECO:0000269|PubMed:2120041}. |
| Signal Peptide | SIGNAL 1..32 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 39,186 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |