| IED ID | IndEnz0011000414 |
| Enzyme Type ID | glucanase000414 |
| Protein Name |
Beta-glucanase EC 3.2.1.73 1,3-1,4-beta-D-glucan 4-glucanohydrolase Endo-beta-1,3-1,4 glucanase Lichenase Mixed linkage beta-glucanase |
| Gene Name | Fisuc_2961 FSU_0226 |
| Organism | Fibrobacter succinogenes (strain ATCC 19169 / S85) |
| Taxonomic Lineage | cellular organisms Bacteria FCB group Fibrobacteres Fibrobacteria Fibrobacterales Fibrobacteraceae Fibrobacter Fibrobacter succinogenes (Bacteroides succinogenes) Fibrobacter succinogenes subsp. succinogenes Fibrobacter succinogenes (strain ATCC 19169 / S85) |
| Enzyme Sequence | MNIKKTAVKSALAVAAAAAALTTNVSAKDFSGAELYTLEEVQYGKFEARMKMAAASGTVSSMFLYQNGSEIADGRPWVEVDIEVLGKNPGSFQSNIITGKAGAQKTSEKHHAVSPAADQAFHTYGLEWTPNYVRWTVDGQEVRKTEGGQVSNLTGTQGLRFNLWSSESAAWVGQFDESKLPLFQFINWVKVYKYTPGQGEGGSDFTLDWTDNFDTFDGSRWGKGDWTFDGNRVDLTDKNIYSRDGMLILALTRKGQESFNGQVPRDDEPAPQSSSSAPASSSSVPASSSSVPASSSSAFVPPSSSSATNAIHGMRTTPAVAKEHRNLVNAKGAKVNPNGHKRYRVNFEH |
| Enzyme Length | 349 |
| Uniprot Accession Number | P17989 |
| Absorption | |
| Active Site | ACT_SITE 79; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10064; ACT_SITE 83; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10064 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.; EC=3.2.1.73; |
| DNA Binding | |
| EC Number | 3.2.1.73 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (18); Chain (1); Compositional bias (1); Domain (1); Helix (5); Region (2); Repeat (5); Signal peptide (1); Turn (4) |
| Keywords | 3D-structure;Direct protein sequencing;Glycosidase;Hydrolase;Reference proteome;Repeat;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..27; /evidence=ECO:0000269|PubMed:2193918 |
| Structure 3D | X-ray crystallography (7) |
| Cross Reference PDB | 1MVE; 1ZM1; 2R49; 3AXD; 3AXE; 3H0O; 3HR9; |
| Mapped Pubmed ID | 12842475; 16246371; 21959377; |
| Motif | |
| Gene Encoded By | |
| Mass | 37,738 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.73; |