Detail Information for IndEnz0011000428
IED ID IndEnz0011000428
Enzyme Type ID glucanase000428
Protein Name Probable glucan endo-1,3-beta-glucosidase btgC
EC 3.2.1.39
Endo-1,3-beta-glucanase btgC
Laminarinase btgC
Gene Name btgC AO090020000436
Organism Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus oryzae (Yellow koji mold) Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Enzyme Sequence MSGPHRSFSFNQGDDGAGDAGDVSPIRSQEGHFMNSPPRHNDVSPVSARSQAMGSSPSSGFLSAHEHGDRGWGQNSGHTQAMRTNSTTPGMDNLGPAAVGGGISGIALGVANSHNRQSGIDAFRDTDGRNLPAERGYNTTGSDNPYVPTPPGGGSHGSAENLRPRDSYGSNVALGAAAAPAGQLTPGGSNPSQRSLFDSPYQGVGAMDAGPYQRQSAYSAAGDYPLVINPDEIADDGDDGFTPVPNGKSASSNARAIPAAAAGGAAGGGLFGLFKSKKADNPSYGPVPGAGLEAGEKSRWVKPTPGGGSRKRGWIVGLALAFIVVGAIVGGAVGGTLGNRENEAPDTTKSASSDTESNGDLNKDSSEIKDLMNNPDLHKVFPGMDYTPWGVQYPLCLKYPPSQNNVTRDVAVLSQLTNTVRLYGTDCNQTEMVLHAIDRLELKDMKVWLGVWIDSNDTTNDRQIKQLYKVLDDTKDISIFKGAIVGNEALYRAGNDIASAKKKLISYMDDVRNHFKEKNYDLPIATSDLGDNWKEDLVTATDLVMSNVHPFFAGVTAKEAAGWTWNFWNQNDVPLTKGTNKKQVISEVGWPSGGGNDCGSNNKCTDDTSGSVAGIDEMNQFMSDWICQALENGTDYFWFEAFDEPWKVQYNTKDENWEDKWGLMDAARKLKPGLKIPDCGGKTAA
Enzyme Length 685
Uniprot Accession Number Q2U492
Absorption
Active Site ACT_SITE 488; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O22317; ACT_SITE 587; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O22317
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.; EC=3.2.1.39;
DNA Binding
EC Number 3.2.1.39
Enzyme Function FUNCTION: Glucanases play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme may be involved in beta-glucan degradation. Active on laminarin and lichenan (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Compositional bias (4); Glycosylation (4); Region (4); Topological domain (2); Transmembrane (1)
Keywords Carbohydrate metabolism;Cell membrane;Cell wall biogenesis/degradation;Glycoprotein;Hydrolase;Membrane;Polysaccharide degradation;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 72,800
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda