Detail Information for IndEnz0011000453
IED ID IndEnz0011000453
Enzyme Type ID glucanase000453
Protein Name Soybean toxin 17 kDa chain
SBTX 17 kDa chain
Cyclophilin-like protein
Fragment
Gene Name
Organism Glycine max (Soybean) (Glycine hispida)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Fabales Fabaceae Papilionoideae 50 kb inversion clade NPAAA clade indigoferoid/millettioid clade Phaseoleae Glycine Glycine subgen. Soja Glycine max (Soybean) (Glycine hispida)
Enzyme Sequence PNPKVFFDMTIGGQSAGRIVMEEYA
Enzyme Length 25
Uniprot Accession Number P86548
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Involved in plant defense (PubMed:18328522). Inhibits spore germination in C.sojina, A.niger (at concentrations >50 ug/ml) and P.herguei but not in F.oxysporum and F.solani (PubMed:20831249, PubMed:18328522). Does not inhibit vegetative mycelial growth (PubMed:20831249). Inhibits growth of C.albicans and K.marxiannus but not P.membranifaciens or C.parapsilosis (PubMed:20831249, PubMed:23894655). Probably acts by affecting the cell membrane (PubMed:20831249). Does not have urease, chitinase, beta-1,3-glucanase or hemagglutination activities (PubMed:18328522, PubMed:20831249). Does not inhibit trypsin (PubMed:18328522). Injection into mice produces toxic effects such as dyspnea, tonic-clonic convulsion and death (PubMed:18328522). {ECO:0000269|PubMed:18328522, ECO:0000269|PubMed:20831249, ECO:0000269|PubMed:23894655}.
Temperature Dependency
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Activity decreases below pH 5.5 and above pH 8.0. {ECO:0000269|PubMed:18328522};
Pathway
nucleotide Binding
Features Chain (1); Non-terminal residue (1)
Keywords Antimicrobial;Direct protein sequencing;Disulfide bond;Fungicide;Plant defense;Reference proteome;Toxin
Interact With
Induction INDUCTION: In seeds, induced by jasmonate. {ECO:0000269|Ref.2}.
Subcellular Location
Modified Residue
Post Translational Modification PTM: SBTX is known to be glycosylated but it is not known which one of its two subunits is modified; contains 5% carbohydrates. {ECO:0000269|PubMed:18328522}.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 2,758
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda