Detail Information for IndEnz0011000476
IED ID IndEnz0011000476
Enzyme Type ID glucanase000476
Protein Name Xyloglucan endotransglucosylase/hydrolase protein 2
At-XTH2
XTH-2
EC 2.4.1.207
Gene Name XTH2 At4g13090 F25G13.180
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MNRIRYCFELVSVLFLMFTANARARGRGAIDFDVNYVVTWGQDHILKLNQGKEVQLSMDYSSGSGFESKSHYGSGFFQMRIKLPPRDSAGVVTAFYLTSKGDTHDEVDFEFLGNRQGKPIAIQTNVFSNGQGGREQKFVPWFDPTTSFHTYGILWNPYQIVFYVDKVPIRVFKNIKKSGVNYPSKPMQLVASLWNGENWATSGGKEKINWAYAPFKAQYQGFSDHGCHVNGQSNNANVCGSTRYWWNTRTYSQLSANEQKVMENVRAKYMTYDYCSDRPRYPVPPSECRWNQ
Enzyme Length 292
Uniprot Accession Number Q9SV60
Absorption
Active Site ACT_SITE 106; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q8GZD5; ACT_SITE 110; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:Q8GZD5
Activity Regulation
Binding Site BINDING 110; /note=Xyloglucan; /evidence=ECO:0000250|UniProtKB:Q8GZD5; BINDING 203; /note=Xyloglucan; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q8GZD5; BINDING 280; /note=Xyloglucan; /evidence=ECO:0000250|UniProtKB:Q8GZD5
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.; EC=2.4.1.207;
DNA Binding
EC Number 2.4.1.207
Enzyme Function FUNCTION: May catalyze xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Binding site (3); Chain (1); Disulfide bond (2); Domain (1); Region (3); Signal peptide (1); Site (1)
Keywords Apoplast;Cell wall;Cell wall biogenesis/degradation;Disulfide bond;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal;Transferase
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000305}. Secreted, extracellular space, apoplast {ECO:0000305}.
Modified Residue
Post Translational Modification PTM: Contains at least one intrachain disulfide bond essential for its enzymatic activity. {ECO:0000250}.
Signal Peptide SIGNAL 1..24; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 11673616; 15720654;
Motif
Gene Encoded By
Mass 33,525
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda