| IED ID | IndEnz0011000477 |
| Enzyme Type ID | glucanase000477 |
| Protein Name |
Inactive glycoside hydrolase XLP1 Glycoside hydrolase family 12 protein XLP1 GH12 protein XLP1 XEG1-like protein 1 |
| Gene Name | XLP1 PPTG_19378 |
| Organism | Phytophthora parasitica (strain INRA-310) |
| Taxonomic Lineage | cellular organisms Eukaryota Sar Stramenopiles Oomycota Peronosporales Peronosporaceae Phytophthora Phytophthora parasitica (Potato buckeye rot agent) Phytophthora parasitica (strain INRA-310) |
| Enzyme Sequence | MKSFLIAIVIAVLLPVSAADFCAQWRLSKAGKYIIYNNLWNQNTATSGSQCTGVDKVSGSTVAWHTSYSWAGAPTQVKSYSNAALVFTKKQIKNIKTIPTTMKYSYSYSGTLIADVAYDLFTSSTASGSNEYEIMIWLAAYGGAGPISSTGKAIATVTINSNSFKLYKGPNGSTTVYSFVATKTITNFSADLLDFFTYLVKTQAFPSSQYLTTLEAGTEPFTGSNAKMTVSSYSAAVN |
| Enzyme Length | 238 |
| Uniprot Accession Number | W2PDG1 |
| Absorption | |
| Active Site | ACT_SITE 133; /evidence=ECO:0000250|UniProtKB:G4ZHR2; ACT_SITE 219; /evidence=ECO:0000250|UniProtKB:G4ZHR2 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Non-functional secreted XEG1-like protein that binds to host Nicotiana benthamiana apoplastic glucanase inhibitor protein GIP2 more tightly than does XEG1, thus it outcompetes XEG1 for GIP2 binding and frees functional XEG1 to support P.parasitica infection (PubMed:28082413). With XEG1, is required to elevate apoplastic sugar during P.parasitica infection (PubMed:28082413). {ECO:0000269|PubMed:28082413}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Glycosylation (2); Mutagenesis (8); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal;Virulence |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:G4ZHR3}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 25,545 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |