| IED ID | IndEnz0011000490 |
| Enzyme Type ID | glucanase000490 |
| Protein Name |
Xyloglucanase Xgh74A EC 3.2.1.- |
| Gene Name | xghA |
| Organism | Acetivibrio thermocellus (Hungateiclostridium thermocellum) (Clostridium thermocellum) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Clostridia Eubacteriales Oscillospiraceae Acetivibrio Acetivibrio thermocellus (Hungateiclostridium thermocellum) (Clostridium thermocellum) |
| Enzyme Sequence | MVKKFTSKIKAAVFAAVVAATAIFGPAISSQAVTSVPYKWDNVVIGGGGGFMPGIVFNETEKDLIYARADIGGAYRWDPSTETWIPLLDHFQMDEYSYYGVESIATDPVDPNRVYIVAGMYTNDWLPNMGAILRSTDRGETWEKTILPFKMGGNMPGRSMGERLAIDPNDNRILYLGTRCGNGLWRSTDYGVTWSKVESFPNPGTYIYDPNFDYTKDIIGVVWVVFDKSSSTPGNPTKTIYVGVADKNESIYRSTDGGVTWKAVPGQPKGLLPHHGVLASNGMLYITYGDTCGPYDGNGKGQVWKFNTRTGEWIDITPIPYSSSDNRFCFAGLAVDRQNPDIIMVTSMNAWWPDEYIFRSTDGGATWKNIWEWGMYPERILHYEIDISAAPWLDWGTEKQLPEINPKLGWMIGDIEIDPFNSDRMMYVTGATIYGCDNLTDWDRGGKVKIEVKATGIEECAVLDLVSPPEGAPLVSAVGDLVGFVHDDLKVGPKKMHVPSYSSGTGIDYAELVPNFMALVAKADLYDVKKISFSYDGGRNWFQPPNEAPNSVGGGSVAVAADAKSVIWTPENASPAVTTDNGNSWKVCTNLGMGAVVASDRVNGKKFYAFYNGKFYISTDGGLTFTDTKAPQLPKSVNKIKAVPGKEGHVWLAAREGGLWRSTDGGYTFEKLSNVDTAHVVGFGKAAPGQDYMAIYITGKIDNVLGFFRSDDAGKTWVRINDDEHGYGAVDTAITGDPRVYGRVYIATNGRGIVYGEPASDEPVPTPPQVDKGLVGDLNGDNRINSTDLTLMKRYILKSIEDLPVEDDLWAADINGDGKINSTDYTYLKKYLLQAIPELPKK |
| Enzyme Length | 842 |
| Uniprot Accession Number | Q70DK5 |
| Absorption | |
| Active Site | ACT_SITE 70; /note=Nucleophile; /evidence=ECO:0000269|PubMed:16772298; ACT_SITE 480; /note=Proton donor; /evidence=ECO:0000269|PubMed:16772298 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.2.1.- |
| Enzyme Function | FUNCTION: Hydrolyzes the glucosidic bonds of unbranched Glc residues in tamarind seed xyloglucan, producing XXXG, XLXG, XXLG and XLLG. Has low activity on carboxymethylcellulose, lichenan,hydroxyethylcellulose and glucuronoxylan, and no activity on xylan, polygalaturonic acid, wheat arabinoxylan, rhamnogalacturan, curdlan, laminarin, galactomannan, galactan, arabinan and pachyman or amorphous cellulose. {ECO:0000269|PubMed:16207921, ECO:0000269|PubMed:16772298}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 75 degrees Celsius. Retains 25% of its activity after incubation at 90 degrees Celsius for 30 minutes. {ECO:0000269|PubMed:16207921}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.4 with tamarind seed xyloglucan as substrate. Retains 50% of its activity between pH 5.7 and 7.8. {ECO:0000269|PubMed:16207921}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (56); Chain (1); Domain (1); Helix (4); Mutagenesis (2); Repeat (9); Signal peptide (1); Turn (5) |
| Keywords | 3D-structure;Carbohydrate metabolism;Cellulose degradation;Glycosidase;Hydrolase;Polysaccharide degradation;Repeat;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..32; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 2CN2; 2CN3; |
| Mapped Pubmed ID | 16127726; |
| Motif | |
| Gene Encoded By | |
| Mass | 92,393 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.155; |