| IED ID | IndEnz0012000001 |
| Enzyme Type ID | hemicellulase000001 |
| Protein Name |
Feruloyl esterase B EC 3.1.1.73 Cinnamoyl ester hydrolase Esterase A EstA Ferulic acid esterase B |
| Gene Name | ESTA |
| Organism | Piromyces equi |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Fungi incertae sedis Chytridiomycota Chytridiomycota incertae sedis Neocallimastigomycetes Neocallimastigales Neocallimastigaceae Piromyces unclassified Piromyces Piromyces equi |
| Enzyme Sequence | MKTSIVLSIVALFLTSKASADCWSERLGWPCCSDSNAEVIYVDDDGDWGVENNDWCGIQKEEENNNSWDMGDWNQGGNQGGGMPWGDFGGNQGGGMQWGDFGGNQGGGMPWGDFGGNQGGGMPWGDFGGNQGGNQGGGMPWGDFGGNQGGNQGGGMPWGDFGGNQGGGMQWGDFGGNQGGNQGGGMPWGDFGGNQGGGMQWGDFGGNQGGNQGGGMPWGDFGGNQGGGMQWGDFGGNQGGGMQWGDFGGNQGGNQDWGNQGGNSGPTVEYSTDVDCSGKTLKSNTNLNINGRKVIVKFPSGFTGDKAAPLLINYHPIMGSASQWESGSQTAKAALNDGAIVAFMDGAQGPMGQAWNVGPCCTDADDVQFTRNFIKEITSKACVDPKRIYAAGFSMGGGMSNYAGCQLADVIAAAAPSAFDLAKEIVDGGKCKPARPFPILNFRGTQDNVVMYNGGLSQVVQGKPITFMGAKNNFKEWAKMNGCTGEPKQNTPGNNCEMYENCKGGVKVGLCTINGGGHAEGDGKMGWDFVKQFSLP |
| Enzyme Length | 536 |
| Uniprot Accession Number | Q9Y871 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by the specific serine esterase inhibitor AEBSF. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Feruloyl-polysaccharide + H(2)O = ferulate + polysaccharide.; EC=3.1.1.73; |
| DNA Binding | |
| EC Number | 3.1.1.73 |
| Enzyme Function | FUNCTION: Involved in degradation of plant cell walls. Hydrolyzes of the feruloyl-arabinose ester bond in arabinoxylans as well as the feruloyl-galactose and feruloyl-arabinose ester bonds in pectin. {ECO:0000269|PubMed:10493932}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (1); Domain (1); Glycosylation (1); Region (4); Repeat (12); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Glycoprotein;Hydrolase;Polysaccharide degradation;Repeat;Secreted;Serine esterase;Signal;Xylan degradation |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 55,540 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.1.1.73; |