| IED ID | IndEnz0012000003 |
| Enzyme Type ID | hemicellulase000003 |
| Protein Name |
Endo-1,4-beta-xylanase A EC 3.2.1.8 1,4-beta-D-xylan xylanohydrolase Xylanase A XYLA |
| Gene Name | XYNA |
| Organism | Piromyces sp. |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Fungi incertae sedis Chytridiomycota Chytridiomycota incertae sedis Neocallimastigomycetes Neocallimastigales Neocallimastigaceae Piromyces unclassified Piromyces Piromyces sp. |
| Enzyme Sequence | MKLFQIFPLLLSLTSVTLAADDFCNATGFQGQSVVSTGHDVKKIGNIDYEQWADGGNNSATFYSDGSFKCNFSNTKDYLCRSGVAFSQAKYPSEIGHIEAEYRLVKKSASNVGYSYVGVYGWTLQSGISGVYEYYIVDNWLSQWRPGDWVGNTKFGDFTIDGGVYTVYKNVNGNLTQYFSLRKSERTCGTIDVTAHFAQWEKLGLKMPKITEIKVLAEAGNTGGGCSGSVEIPYAKIYINGKDQDGKSKGGSSSGGSNGQGLGNGQGNGQGQGNGQGQSATGSGKCPSTITSQGYKCCSSNCDIIYRDQSGDWGVENDEWCGCGSRVPKTTNCPSSIKNQGYKCCSDSCEIVLTDSDGDWGIENDEWCGCGIKNTTPTTTTKKSNNSQPTQGQSNNNSSTNTNFCSTSKHSGQSVTETSNKVGSIGGVGYELWADSGNNSATFYSDGSFSCSFRNAKDYLCRSGLSFDSTKTYQQLGHMYADFKLVKQNIQNVDYSYVGIYGWTRNPLVEFYVVDNWLSQWRPGDWVGNKKHGDFTIDGAKYTVYENTRTGPSIDGNTTFKQYFSIRQQARDCGTIDITAHFEQWEKLGMRMGKMHEAKVLGEAGSTGSGTSGTADFPYAKVYIK |
| Enzyme Length | 625 |
| Uniprot Accession Number | Q12667 |
| Absorption | |
| Active Site | ACT_SITE 510; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10062; ACT_SITE 603; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; |
| DNA Binding | |
| EC Number | 3.2.1.8 |
| Enzyme Function | FUNCTION: Hydrolyzes 1,4-beta linked polysaccharide backbones of xylans, one of the major hemicellulose components in hardwoods and softwoods. It is more active against xylopentaose than xylotetraose, has trace activity against xylotriose. The major products released from hydrolysis of xylooligosaccharides are xylobiose and xylotriose. The reiterated 40 AA domain is involved in binding the cellulase-hemicellulase complex. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan degradation; xylan degradation. |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (1); Domain (4); Region (5); Repeat (2); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Glycosidase;Hydrolase;Polysaccharide degradation;Repeat;Signal;Xylan degradation |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 68,049 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |