| IED ID | IndEnz0015000013 |
| Enzyme Type ID | laccase000013 |
| Protein Name |
Laccase-2 EC 1.10.3.2 Benzenediol:oxygen oxidoreductase 2 Diphenol oxidase 2 Laccase I Urishiol oxidase 2 |
| Gene Name | LCC2 LCCI |
| Organism | Trametes versicolor (White-rot fungus) (Coriolus versicolor) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Agaricomycetes Agaricomycetes incertae sedis Polyporales Polyporaceae (bracket fungi) Trametes Trametes versicolor (White-rot fungus) (Coriolus versicolor) |
| Enzyme Sequence | MGLQRFSFFVTLALVARSLAAIGPVASLVVANAPVSPDGFLRDAIVVNGVVPSPLITGKKGDRFQLNVDDTLTNHSMLKSTSIHWHGFFQAGTNWADGPAFVNQCPIASGHSFLYDFHVPDQAGTFWYHSHLSTQYCDGLRGPFVVYDPKDPHASRYDVDNESTVITLTDWYHTAARLGPRFPLGADATLINGLGRSASTPTAALAVINVQHGKRYRFRLVSISCDPNYTFSIDGHNLTVIEVDGINSQPLLVDSIQIFAAQRYSFVLNANQTVGNYWVRANPNFGTVGFAGGINSAILRYQGAPVAEPTTTQTTSVIPLIETNLHPLARMPVPGSPTPGGVDKALNLAFNFNGTNFFINNATFTPPTVPVLLQILSGAQTAQDLLPAGSVYPLPAHSTIEITLPATALAPGAPHPFHLHGHAFAVVRSAGSTTYNYNDPIFRDVVSTGTPAAGDNVTIRFQTDNPGPWFLHCHIDFHLDAGFAIVFAEDVADVKAANPVPKAWSDLCPIYDGLSEANQ |
| Enzyme Length | 519 |
| Uniprot Accession Number | Q12718 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O; Xref=Rhea:RHEA:11276, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:17594, ChEBI:CHEBI:17977; EC=1.10.3.2; Evidence={ECO:0000250|UniProtKB:Q70KY3}; |
| DNA Binding | |
| EC Number | 1.10.3.2 |
| Enzyme Function | FUNCTION: Lignin degradation and detoxification of lignin-derived products. {ECO:0000250|UniProtKB:Q70KY3}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (34); Chain (1); Disulfide bond (2); Domain (3); Glycosylation (8); Helix (14); Metal binding (11); Sequence conflict (1); Signal peptide (1); Turn (3) |
| Keywords | 3D-structure;Copper;Disulfide bond;Glycoprotein;Lignin degradation;Metal-binding;Oxidoreductase;Repeat;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:12163489}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 1GYC; |
| Mapped Pubmed ID | 11825613; |
| Motif | |
| Gene Encoded By | |
| Mass | 55,811 |
| Kinetics | |
| Metal Binding | METAL 84; /note="Copper 1; type 2"; /evidence="ECO:0000269|PubMed:12163489, ECO:0007744|PDB:1GYC"; METAL 86; /note="Copper 2; type 3"; /evidence="ECO:0000269|PubMed:12163489, ECO:0007744|PDB:1GYC"; METAL 129; /note="Copper 2; type 3"; /evidence="ECO:0000269|PubMed:12163489, ECO:0007744|PDB:1GYC"; METAL 131; /note="Copper 3; type 3"; /evidence="ECO:0000269|PubMed:12163489, ECO:0007744|PDB:1GYC"; METAL 415; /note="Copper 4; type 1"; /evidence="ECO:0000269|PubMed:12163489, ECO:0007744|PDB:1GYC"; METAL 418; /note="Copper 1; type 2"; /evidence="ECO:0000269|PubMed:12163489, ECO:0007744|PDB:1GYC"; METAL 420; /note="Copper 3; type 3"; /evidence="ECO:0000269|PubMed:12163489, ECO:0007744|PDB:1GYC"; METAL 472; /note="Copper 3; type 3"; /evidence="ECO:0000269|PubMed:12163489, ECO:0007744|PDB:1GYC"; METAL 473; /note="Copper 4; type 1"; /evidence="ECO:0000269|PubMed:12163489, ECO:0007744|PDB:1GYC"; METAL 474; /note="Copper 2; type 3"; /evidence="ECO:0000269|PubMed:12163489, ECO:0007744|PDB:1GYC"; METAL 478; /note="Copper 4; type 1"; /evidence="ECO:0000269|PubMed:12163489, ECO:0007744|PDB:1GYC" |
| Rhea ID | RHEA:11276 |
| Cross Reference Brenda | 1.10.3.2; |