Detail Information for IndEnz0015000016
IED ID IndEnz0015000016
Enzyme Type ID laccase000016
Protein Name Laccase-1
EC 1.10.3.2
Diphenol oxidase 1
Gene Name LAC1 CNBG3550
Organism Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) (Filobasidiella neoformans)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Tremellomycetes Tremellales (jelly fungi) Cryptococcaceae Cryptococcus Cryptococcus neoformans species complex Cryptococcus neoformans (Filobasidiella neoformans) Cryptococcus neoformans var. neoformans Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) (Filobasidiella neoformans)
Enzyme Sequence MRGLAKLFFLSCSFVSLVSSEKTDESPTAVSDNYMPKATATIDPSVFALSNDFEITDVPTTREYTFDIAKAFASPDGYEREVYVVNNMFPGPVIEANTGDTIIVHVNNHLDEGQSLHWHGLRQLGTAFMDGVPGITQCPIPPGGSFTYNFTVSHQSGTYWWHSHYSNSMADGIWGPLIVHSPNEPLQRGRDYDEDRIVFITDWMHDNSEIIIAALATPEGYKGNIAPPQGDAILINGRGQTNCTATGSSSCFYPPPPEIQVPVNCRVRLRFISATAHPMYRISIDNHPMEVVEADGTAVYGPTVHEISVAPGERYSAIINTNEGKEGDAFWLRTSVALSCMFGAVSQEGLAVVRYTGNGMVSTEEPQTSAWSDLAGVTVPCTGLDQTYTLSPRDSLSAPREPLQSHFFNSERGAFVNVLGNTFQGYGFNNISYQNQIFNPLLSIVQRGGSCENTLVSSRTFPDFGPGNIIINNLDTVIDHPYHLHGNEFQVIGRGTGALSIDNLTNIDFTLDNPVRKDTLWIQGGSWAVLRITADNPGVWALHCHIGWHLTEGKLAVIVVQPSAIGHMESPESWTNLCANTDPNAFGPAKRSSSPSIQSSKTSSFQYLREVKGKVVKRRGAREA
Enzyme Length 624
Uniprot Accession Number Q55P57
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O; Xref=Rhea:RHEA:11276, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:17594, ChEBI:CHEBI:17977; EC=1.10.3.2; Evidence={ECO:0000269|PubMed:8300520};
DNA Binding
EC Number 1.10.3.2
Enzyme Function FUNCTION: Laccase that catalyzes the oxidation of certain aromatic compounds, including L-dopa, to quinones, which then polymerize to melanin (PubMed:1100669, PubMed:6807845, PubMed:6804444, PubMed:8760791). Able to oxidize a wide variety of aromatic diphenol and diamino groups in the ortho, meta, and para positions but not monophenolic groups such as in phenol, tyramine, or tyrosine (PubMed:8300520). Plays an important role in virulence (PubMed:8760791). Plays a role in dissemination to extrapulmonary sites but is not involved in pulmonary growth or in elicitation of cellular immune responses in the lung (PubMed:14977977). {ECO:0000269|PubMed:1100669, ECO:0000269|PubMed:14977977, ECO:0000269|PubMed:6804444, ECO:0000269|PubMed:6807845, ECO:0000269|PubMed:8300520, ECO:0000269|PubMed:8760791}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Disulfide bond (1); Domain (3); Glycosylation (4); Metal binding (11); Mutagenesis (1); Region (1); Signal peptide (1)
Keywords Cell wall;Copper;Direct protein sequencing;Disulfide bond;Glycoprotein;Metal-binding;Oxidoreductase;Repeat;Secreted;Signal
Interact With
Induction INDUCTION: Expressed during infection of host (PubMed:8760791). Expression is repressed by glucose (PubMed:8300520). {ECO:0000269|PubMed:8300520, ECO:0000269|PubMed:8760791}.
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11500433}. Secreted, cell wall {ECO:0000269|PubMed:11500433}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..20; /evidence=ECO:0000269|PubMed:8300520
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 68,270
Kinetics
Metal Binding METAL 117; /note=Copper 1; type 2; /evidence=ECO:0000250|UniProtKB:D0VWU3; METAL 119; /note=Copper 2; type 3; /evidence=ECO:0000250|UniProtKB:D0VWU3; METAL 162; /note=Copper 2; type 3; /evidence=ECO:0000250|UniProtKB:D0VWU3; METAL 164; /note=Copper 3; type 3; /evidence=ECO:0000250|UniProtKB:D0VWU3; METAL 480; /note=Copper 4; type 1; /evidence=ECO:0000250|UniProtKB:D0VWU3; METAL 483; /note=Copper 1; type 2; /evidence=ECO:0000250|UniProtKB:D0VWU3; METAL 485; /note=Copper 3; type 3; /evidence=ECO:0000250|UniProtKB:D0VWU3; METAL 543; /note=Copper 3; type 3; /evidence=ECO:0000250|UniProtKB:D0VWU3; METAL 544; /note=Copper 4; type 1; /evidence=ECO:0000250|UniProtKB:D0VWU3; METAL 545; /note=Copper 2; type 3; /evidence=ECO:0000250|UniProtKB:D0VWU3; METAL 549; /note=Copper 4; type 1; /evidence=ECO:0000250|UniProtKB:D0VWU3
Rhea ID RHEA:11276
Cross Reference Brenda