| IED ID | IndEnz0015000022 |
| Enzyme Type ID | laccase000022 |
| Protein Name |
Laccase-2 EC 1.10.3.2 Benzenediol:oxygen oxidoreductase 2 Diphenol oxidase 2 Urishiol oxidase 2 |
| Gene Name | LAC2 At2g29130 T9I4.21 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MVTWVLNYLLVAFLFAISYNIDAASAGITRHYQFDIQLKNITRLCKTKTIVTVNGKFPGPRVTAREGDNLQIKVVNHVSNNISIHWHGIRQLRSGWADGPSYVTQCPIRMGQSYVYNFTVTGQRGTLWWHAHIQWMRATVYGPLIILPKLHQPYPFPKPYKQVPILFGEWFNADPQAVVQQALQTGAGPNASDAHTFNGLPGPLYNCSTKDTYKLMVKPGKTYLLRLINAALNDELFFTIANHTLTVVEADACYVKPFQTNIVLLGPGQTTNVLLKTKPIYPNATFYMLARPYFTGQGTIDNTTVAGILQYQHHTKSSKNLSIIKPSLPPINSTSYAANFTKMFRSLASSTFPANVPKVVDKQYFFAIGLGTNPCPKNQTCQGPTNTTKFAASINNVSFILPNKTSLLQSYFVGKSKNVFMTDFPTAPIIPFNYTGTPPNNTMVSRGTKVVVLKYKTTVELVLQGTSILGIEAHPIHLHGFNFYVVGQGFGNFNPARDPKHYNLVDPVERNTINIPSGGWVAIRFLADNPGVWLMHCHIEIHLSWGLTMAWVVLDGDLPNQKLLPPPSDFPKC |
| Enzyme Length | 573 |
| Uniprot Accession Number | O81081 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O; Xref=Rhea:RHEA:11276, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:17594, ChEBI:CHEBI:17977; EC=1.10.3.2; |
| DNA Binding | |
| EC Number | 1.10.3.2 |
| Enzyme Function | FUNCTION: Lignin degradation and detoxification of lignin-derived products (By similarity). Required for root elongation in dehydration conditions. {ECO:0000250, ECO:0000269|PubMed:16804053}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Domain (3); Glycosylation (17); Metal binding (11); Signal peptide (1) |
| Keywords | Apoplast;Copper;Glycoprotein;Lignin degradation;Metal-binding;Oxidoreductase;Reference proteome;Repeat;Secreted;Signal |
| Interact With | |
| Induction | INDUCTION: By NaCl and 20% PEG. {ECO:0000269|PubMed:16804053}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..26; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10394942; 15258262; 15937231; 15980264; 16243908; 16367961; 18408011; 18467456; 18480120; 18723362; 19582540; 21063888; 21069430; 21447792; 31949029; 32127430; 9914531; |
| Motif | |
| Gene Encoded By | |
| Mass | 63,832 |
| Kinetics | |
| Metal Binding | METAL 85; /note=Copper 1; type 2; /evidence=ECO:0000250; METAL 87; /note=Copper 2; type 3; /evidence=ECO:0000250; METAL 130; /note=Copper 2; type 3; /evidence=ECO:0000250; METAL 132; /note=Copper 3; type 3; /evidence=ECO:0000250; METAL 474; /note=Copper 4; type 1; /evidence=ECO:0000250; METAL 477; /note=Copper 1; type 2; /evidence=ECO:0000250; METAL 479; /note=Copper 3; type 3; /evidence=ECO:0000250; METAL 536; /note=Copper 3; type 3; /evidence=ECO:0000250; METAL 537; /note=Copper 4; type 1; /evidence=ECO:0000250; METAL 538; /note=Copper 2; type 3; /evidence=ECO:0000250; METAL 542; /note=Copper 4; type 1; /evidence=ECO:0000250 |
| Rhea ID | RHEA:11276 |
| Cross Reference Brenda |