| IED ID | IndEnz0015000024 |
| Enzyme Type ID | laccase000024 |
| Protein Name |
Laccase EC 1.10.3.2 Benzenediol:oxygen oxidoreductase Diphenol oxidase Urishiol oxidase Fragment |
| Gene Name | |
| Organism | Lepiota magnispora (Mushroom) (Lepiota ventriosospora) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Agaricomycetes Agaricomycetidae Agaricales Agaricaceae Lepiota Lepiota magnispora (Mushroom) (Lepiota ventriosospora) |
| Enzyme Sequence | VTIGKEGTLT |
| Enzyme Length | 10 |
| Uniprot Accession Number | B3EWG2 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by NaN(3) and SDS. Partially inhibited by Fe(2+), Fe(3+) and Hg(2+) but not by other metal ions at a concentration of 10 mM. {ECO:0000269|Ref.1}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O; Xref=Rhea:RHEA:11276, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:17594, ChEBI:CHEBI:17977; EC=1.10.3.2; Evidence={ECO:0000269|Ref.1}; |
| DNA Binding | |
| EC Number | 1.10.3.2 |
| Enzyme Function | FUNCTION: Lignin degradation and detoxification of lignin-derived products (By similarity). In vitro, has activity towards 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), catechol and hydroquinone but not towards 2,6-dimethoxy-phenol or guaiacol. {ECO:0000250|UniProtKB:P86351, ECO:0000269|Ref.1}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 60 degrees Celsius. After incubation at 80 degrees Celsius or 20 degrees Celsius for 5 minutes 50% activity remains. Activity is lost after incubation at 100 degrees Celsius for 5 minutes. After incubation at 60 degrees Celsius for 1 hour 18% activity remains. {ECO:0000269|Ref.1}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4. Activity decreases at higher pH and is lost at pH 6. {ECO:0000269|Ref.1}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Non-terminal residue (1) |
| Keywords | Copper;Direct protein sequencing;Lignin degradation;Metal-binding;Oxidoreductase;Secreted |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P86351}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 1,018 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=54.9 uM for ABTS (at pH 4) {ECO:0000269|Ref.1}; KM=10.2 uM for catechol (at pH 4) {ECO:0000269|Ref.1}; KM=12.6 uM for hydroquinone (at pH 4) {ECO:0000269|Ref.1}; |
| Metal Binding | |
| Rhea ID | RHEA:11276 |
| Cross Reference Brenda |