| IED ID | IndEnz0015000042 |
| Enzyme Type ID | laccase000042 |
| Protein Name |
Laccase 1 EC 1.10.3.- Conidial pigment biosynthesis oxidase Mlac1 |
| Gene Name | Mlac1 Abr2 MAN_10477 |
| Organism | Metarhizium anisopliae (strain ARSEF 549) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Clavicipitaceae Metarhizium Metarhizium anisopliae (Entomophthora anisopliae) Metarhizium anisopliae (strain ARSEF 549) |
| Enzyme Sequence | MSRFARLLLIVALFFTSAWAKTVKETLRITWEEGAPNGQARELIYTNGQFPSPTLVWDEDDDIEVTVYNEMAKNVTVHWHGLDQKDTPWSDGTPGLSQRPIQPGNKFVYRFKASPPGNHWYHSHEKMSLVDGLYGAIHIRPKGDRTGLWSQISQDKDDIKAMENAAYDPEYLVVSDWSQYTSEEYWKISTDSGLLVFCLDSILVNGKGEVYCPGQKFLQAELAPGLVEDAFPPGTEVSDKGCFPADLDQVQGGPWNITKRPDLIPPRVQEGCVASRHENATIVVDPSKNNGWVSMHFVAAATIAQITFSVDSHEFWLYEIDGNYVNPRKFVSAVMSAGETFSVMIKLDQEPGKYTMRIPNSGASQVLGGFAEMVYKGCEHEEKAGKAYLSYGGNPTSPDVEKNSFFPWQLDTDHMSPWPPNKPRPGNADEEHLLVLGRVGAPYNYTMNTKYLYPVDFQNDDPLLFYPNATRDTENDGLVLRTKNGSWVDLILQVSTLPGDTSSFEHFMHKHGSKTWRIGFGTGVWNYTSVEEAIQERPQDFNLETPGLRDTWITAFSIGGEAYWSVFRYFVDNPGPWLFHCHIELHLMGGMGIAILDGVDAWPEHIPEEYQC |
| Enzyme Length | 612 |
| Uniprot Accession Number | A0A0B4F1I0 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 1.10.3.- |
| Enzyme Function | FUNCTION: Laccase; part of the Pks1 gene cluster that mediates the biosynthesis of an anthraquinone derivative pigment that contributes to conidial pigmentation that provides protection from UV radiation, heat and cold stress (PubMed:20382249, PubMed:29958281). The polyketide synthase Pks1 produces 1-acetyl-2,4,6,8-tetrahydroxy-9,10-anthraquinone though condensation of acetyl-CoA with malonyl-CoA (Probable). The dehydratase EthD and the laccase Mlac1 further convert the anthraquinone derivative into the final conidial pigment (PubMed:20382249) (Probable). {ECO:0000269|PubMed:20382249, ECO:0000269|PubMed:29958281, ECO:0000305|PubMed:29958281}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Pigment biosynthesis. {ECO:0000269|PubMed:20382249}. |
| nucleotide Binding | |
| Features | Chain (1); Domain (3); Glycosylation (7); Metal binding (12); Signal peptide (1) |
| Keywords | Copper;Glycoprotein;Metal-binding;Oxidoreductase;Repeat;Signal |
| Interact With | |
| Induction | INDUCTION: Expressed during isotropic growth (swelling) but not during polarized growth (germ tubes and hyphae) (PubMed:20382249). Expressed exclusively in the later stages of conidiation and in blastospores when M.anisopliae is living as a saprophyte (PubMed:20382249). During infection processes, is also expressed by appressoria on the cuticle surface and hyphal bodies inside the insect haemocoel (PubMed:20382249). {ECO:0000269|PubMed:20382249}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cell surface {ECO:0000250|UniProtKB:E9RBR0}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 68,949 |
| Kinetics | |
| Metal Binding | METAL 78; /note=Copper 1; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 80; /note=Copper 2; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 122; /note=Copper 2; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 124; /note=Copper 3; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 506; /note=Copper 4; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 509; /note=Copper 1; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 509; /note=Copper 4; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 511; /note=Copper 3; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 580; /note=Copper 3; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 581; /note=Copper 4; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 582; /note=Copper 2; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 586; /note=Copper 4; /evidence=ECO:0000250|UniProtKB:Q70KY3 |
| Rhea ID | |
| Cross Reference Brenda |