| IED ID | IndEnz0015000045 |
| Enzyme Type ID | laccase000045 |
| Protein Name |
Laccase 1 EC 1.10.3.- Conidial pigment biosynthesis oxidase Mlac1 |
| Gene Name | Mlac1 Abr2 MAM_08216 |
| Organism | Metarhizium album (strain ARSEF 1941) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Clavicipitaceae Metarhizium Metarhizium album Metarhizium album (strain ARSEF 1941) |
| Enzyme Sequence | MDHFARVSLVAALLYTNTWAKTVRETLRITWEEGAPNGQQRELIYINGRFPGPNLVWDEDDDVEVTVINDMTQSVTVHWHGLDQRPIQPGDSFVHKFKAFPPGNHWYHSHQKMSLVDGLYGAVHVRPKGDRKGLWSQISQDDKDIEAMEKAACDPEYLVVSDWSQYTSDEYWKISNDSGLPVFCLDSILVNGKGEVYCPGQKFLQGELAPGVLDAFPPGTEVSDKGCFSPALDRIKGGPWNMTERPDLIPPHVETGCVASRHENETIVVDPGRNNGWVSMHIVAAATIAQIAISFDSHKFWLYEVDGNYVNPREYFSAIISAGETFSIMMKLDQEPGRYTVRIPNTGASQVFSAFAEMVYKGHEENDKKLGEARLSYGGVPTSPEIKNNSYFPWKLDTDHMSPWPPSTPRPGNADEEHLLVLGRVGSPNNHTMNAKYLYPLGFRDEEPLLFYPNATLGTENEGLLLRTRNASWVDLIMQVSTLAGDEVAFKHFIHKHGGKTWRIGFGTGVWNYSSVQEAIQARPNDFNLETPGFRDTWITAPSASGEKHWSVLRYYVDNPGPWLLHCHIELHLMGGMGMVIMDGVDAWPDQLPEQYRLGKRL |
| Enzyme Length | 602 |
| Uniprot Accession Number | A0A0B2WJN5 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 1.10.3.- |
| Enzyme Function | FUNCTION: Laccase; part of the Pks1 gene cluster that mediates the biosynthesis of an anthraquinone derivative pigment that contributes to conidial pigmentation that provides protection from UV radiation, heat and cold stress (PubMed:29958281). The polyketide synthase Pks1 produces 1-acetyl-2,4,6,8-tetrahydroxy-9,10-anthraquinone though condensation of acetyl-CoA with malonyl-CoA (Probable). The dehydratase EthD and the laccase Mlac1 further convert the anthraquinone derivative into the final conidial pigment (Probable). {ECO:0000269|PubMed:29958281, ECO:0000305|PubMed:29958281}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Pigment biosynthesis. {ECO:0000305|PubMed:29958281}. |
| nucleotide Binding | |
| Features | Chain (1); Domain (3); Glycosylation (8); Metal binding (12); Signal peptide (1) |
| Keywords | Copper;Glycoprotein;Metal-binding;Oxidoreductase;Reference proteome;Repeat;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell surface {ECO:0000250|UniProtKB:E9RBR0}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 67,773 |
| Kinetics | |
| Metal Binding | METAL 78; /note=Copper 1; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 80; /note=Copper 2; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 108; /note=Copper 2; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 110; /note=Copper 3; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 492; /note=Copper 4; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 495; /note=Copper 1; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 495; /note=Copper 4; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 497; /note=Copper 3; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 566; /note=Copper 3; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 567; /note=Copper 4; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 568; /note=Copper 2; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 572; /note=Copper 4; /evidence=ECO:0000250|UniProtKB:Q70KY3 |
| Rhea ID | |
| Cross Reference Brenda |