| IED ID | IndEnz0015000064 |
| Enzyme Type ID | laccase000064 |
| Protein Name |
Laccase-25 EC 1.10.3.2 Benzenediol:oxygen oxidoreductase 25 Diphenol oxidase 25 Urishiol oxidase 25 |
| Gene Name | LAC25 Os12g0259800 LOC_Os12g15920 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MTLHWSLLLFIAIALVSSVAQAAVVEHTFNVGNFSISQLCQPPLIITAVNGQLPGPTIYAREGDTVVVHLVNTSPYSMTLHWHGVLQRGTPWADGPAMVTQCPVQPGGNYTYRFNVDGQEGTLWWHAHVSFHRATVYGALVIRPRGGAKAYPFPKPDKEHVVILGEWWNATVYDMERMAFLTGIPAPHADAYTINGKPGDFYNCSAPNQTAKFEVRQNGTYLLRIINAGMNTPLFFKVAKHRLTVVGADACYTKPYKTDVVVVSPGQTVDALMVASAAVGRYYMAASPYDSAIPQGPPFSDTTATAILQYAGARRKTVRWRPPVLPRRPPVNDTATAHRFFSGMTALLRHGKPSAVPLAVDTHMYVTVGLGVSLCQPEQLLCNRSAPPVFSSSMNNASFVVPKNTSLLEAHFRREPAGVYTRDFPDTPPVVFDYTGDESDNATMQFTTKSTKVKTLRYNETVEMVLQNTRLIAKESHPMHIHGFNFFILAQGFGNYDKRRAERRFNLVDPQERNTIAVPTGGWAVIRFVADNPGMWYMHCHFDAHISLGLAMVLEVLDGPTPETSVPPPPADLPRCS |
| Enzyme Length | 577 |
| Uniprot Accession Number | Q0IP28 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O; Xref=Rhea:RHEA:11276, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:17594, ChEBI:CHEBI:17977; EC=1.10.3.2; |
| DNA Binding | |
| EC Number | 1.10.3.2 |
| Enzyme Function | FUNCTION: Lignin degradation and detoxification of lignin-derived products. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Domain (3); Erroneous gene model prediction (1); Glycosylation (12); Metal binding (11); Signal peptide (1) |
| Keywords | Apoplast;Copper;Glycoprotein;Lignin degradation;Metal-binding;Oxidoreductase;Reference proteome;Repeat;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..22; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 63,680 |
| Kinetics | |
| Metal Binding | METAL 81; /note=Copper 1; /evidence=ECO:0000250; METAL 83; /note=Copper 2; /evidence=ECO:0000250; METAL 126; /note=Copper 2; /evidence=ECO:0000250; METAL 128; /note=Copper 3; /evidence=ECO:0000250; METAL 477; /note=Copper 4; /evidence=ECO:0000250; METAL 480; /note=Copper 1; /evidence=ECO:0000250; METAL 482; /note=Copper 3; /evidence=ECO:0000250; METAL 539; /note=Copper 3; /evidence=ECO:0000250; METAL 540; /note=Copper 4; /evidence=ECO:0000250; METAL 541; /note=Copper 2; /evidence=ECO:0000250; METAL 545; /note=Copper 4; /evidence=ECO:0000250 |
| Rhea ID | RHEA:11276 |
| Cross Reference Brenda |