| IED ID | IndEnz0015000076 |
| Enzyme Type ID | laccase000076 |
| Protein Name |
Laccase EC 1.10.3.2 Fragment |
| Gene Name | |
| Organism | Agaricus placomyces (Mushroom) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Agaricomycetes Agaricomycetidae Agaricales Agaricaceae Agaricus Agaricus placomyces (Mushroom) |
| Enzyme Sequence | DVIGPQAQVTLANQD |
| Enzyme Length | 15 |
| Uniprot Accession Number | B3EWI3 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Activity is enhanced by Al(3+) at concentrations of 1.25-2.5 mM (PubMed:23093860). Activity is reduced in the presence of Cu(2+), Hg(2+), Pb(2+), Fe(3+) and EDTA at concentrations of 1.25-5.0 mM (PubMed:23093860). Activity is not significantly affected by the presence of cations such as K(+), Ca(2+), Mg(2+), Mn(2+) or Zn(2+) at concentrations of 1.25-5.0 mM (PubMed:23093860). {ECO:0000269|PubMed:23093860}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O; Xref=Rhea:RHEA:11276, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:17594, ChEBI:CHEBI:17977; EC=1.10.3.2; Evidence={ECO:0000269|PubMed:23093860}; |
| DNA Binding | |
| EC Number | 1.10.3.2 |
| Enzyme Function | FUNCTION: Lignin degradation and detoxification of lignin-derived products (By similarity). Active against a variety of substrates including the benzenediols hydroquinone and catechol, the benzenetriol pyrogallol, the methoxy-substituted phenol 2-methylcatechol, the aromatic diamine N,N-dimethyl-1,4-phenylenediamine and the nonphenolic heterocyclic compound ABTS (PubMed:23093860). Shows highest degradative activity towards hydroquinone with lower activity against ABTS and N,N-dimethyl-1,4-phenylenediamine (PubMed:23093860). Activity towards pyrogallol, catechol and 2-methyl-catechol is considerably attenuated (PubMed:23093860). Shows no activity against tyrosine (PubMed:23093860). {ECO:0000250|UniProtKB:P86351, ECO:0000269|PubMed:23093860}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 30 degrees Celsius. Activity declines sharply as the temperature is increased from 40 to 80 degrees Celsius and is abolished at 80 degrees Celsius. {ECO:0000269|PubMed:23093860}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.2 with ABTS as substrate. Optimum pH is 6.8 with hydroquinone as substrate. {ECO:0000269|PubMed:23093860}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Non-terminal residue (1) |
| Keywords | Copper;Direct protein sequencing;Lignin degradation;Metal-binding;Oxidoreductase;Secreted |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q12718}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 1,569 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.392 mM for hydroquinone {ECO:0000269|PubMed:23093860}; KM=0.775 mM for ABTS {ECO:0000269|PubMed:23093860}; |
| Metal Binding | |
| Rhea ID | RHEA:11276 |
| Cross Reference Brenda |