| IED ID | IndEnz0015000103 |
| Enzyme Type ID | laccase000103 |
| Protein Name |
Laccase PFICI_06862 EC 1.10.3.- Conidial pigment biosynthesis oxidase PFICI_06862 |
| Gene Name | PFICI_06862 |
| Organism | Pestalotiopsis fici (strain W106-1 / CGMCC3.15140) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Xylariomycetidae Xylariales Sporocadaceae Pestalotiopsis Pestalotiopsis fici Pestalotiopsis fici (strain W106-1 / CGMCC3.15140) |
| Enzyme Sequence | MYIQTQFASLLLLAGTSLASQVRKYNFTITSQWSSGDGHGRPVFMINGQSPGPLIEADEGDEIEVFVDNQLAAETTMHWHGIYQIDRPWNDGVPGVTQYSMQPRDTYTYRFTVQQQYGSYFYHGHFGPAFADGMRGPMWIAPAAWRPRPYELISDSSHDVEQMKKAEKHPFHVVISDWNAEPMDILLVMYRDTGIVPWCSNSIVLNGKGRTYCHSAELIESVGGPGRNTLGCLMQPDQELYSNEQVCEATQTDLEIFQAEEGHEWIWINFIHSGAHHELQISVDEHEIVVVAADGEFTYPQRVHAANCNLGERISILVHLNQKPGDYAIRVTSLRQEQVIQGLGILRYPGSSHGAQEAEPPATKPWVHLNGTLISDKLQQMDEMKLAPFPSRPPPPQSDHTLKFFVNMTGTGSWALNIGPHQAFRQQLPPLLWEEDSRGVTTYESDVQGGSMQNGSVVDIIFTNGANVNSQHPFHKHNNKAWVIGTGTGGFPWDTVDEAIQQGGMADSFNFVDPPIRDGCRLGNTTGDWTVIRYDIAFPAASMLHCHMIHHFGAGQQVVLLEGVESMAKIPAEMKDRVHSNFRPPLRYGPLD |
| Enzyme Length | 592 |
| Uniprot Accession Number | W3X732 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 1.10.3.- |
| Enzyme Function | FUNCTION: Laccase involved the biosynthesis of dihydroxynaphthalene (DHN)-melanin, a bluish-green pigment forming a dark layer in the conidial wall that protects the conidia from UV radiations (PubMed:28517364). The first step of the pathway is the production of the pentaketide 1,3,6,8-tetrahydroxynaphthalene (1,3,6,8-THN or T4HN) by the polyketide synthase PfmaE though condensation of acetyl-CoA with malonyl-CoA. T4HN is not stable and easily oxidizes into the stable form flaviolin (PubMed:28517364). T4HN is also substrate of the hydroxynaphthalene reductase PfmaG to yield scytalone (PubMed:28517364). The scytalone dehydratase PfmaJ then reduces scytalone to 1,3,8-THN (PubMed:31116900). 1,3,8-THN is then substrate of the hydroxynaphthalene reductase PfmaI to yield vermelone (Probable). Vermelone is further converted by the multicopper oxidase PfmaD to 1,8-DHN (Probable). Finally the laccase PFICI_06862 transforms 1,8-DHN to DHN-melanin (Probable). The roles of the 5-oxoprolinase PfmaA and the proline iminopeptidase PfmaB within the cluster have not been elucidated yet (Probable). {ECO:0000269|PubMed:28517364, ECO:0000269|PubMed:31116900, ECO:0000305|PubMed:28517364, ECO:0000305|PubMed:31116900}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Pigment biosynthesis; melanin biosynthesis. {ECO:0000305|PubMed:28517364}. |
| nucleotide Binding | |
| Features | Chain (1); Domain (3); Glycosylation (5); Metal binding (5); Signal peptide (1) |
| Keywords | Copper;Glycoprotein;Melanin biosynthesis;Metal-binding;Oxidoreductase;Reference proteome;Repeat;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell surface {ECO:0000250|UniProtKB:E9RBR0}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 66,213 |
| Kinetics | |
| Metal Binding | METAL 78; /note=Copper 1; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 80; /note=Copper 2; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 123; /note=Copper 2; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 125; /note=Copper 3; /evidence=ECO:0000250|UniProtKB:Q70KY3; METAL 475; /note=Copper 4; /evidence=ECO:0000250|UniProtKB:Q70KY3 |
| Rhea ID | |
| Cross Reference Brenda |